摘要
目的:建立高效液相色谱法测定血塞通胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1的含量方法;方法:色谱柱为Di-amonsil C18柱(5μm,250mm×4.6mm);乙腈-水线性梯度洗脱;流速:1.0ml.min-1;检测波长:203nm;柱温:30℃。结果:三七皂苷R1、人参皂苷Rgl和人参皂苷Rb1的线性范围分别为2.555～12.775μg、8.125～40.625μg和7.665～38.325μg,相关系数分别为0.9996、0.9999和0.998,平均加样回收率(n=6)分别为99.2%、99.8%和99.5%,RSD分别为0.52%、0.28%和0.45%。结论:本方法具有简便、快速、准确等特点,可用于血塞通胶囊的质量控制。
Objective： The method for the determination of notoginsenoside R1, ginsenoside Rgl and ginsenoside Rbl in Xuesaitong Capusle was established. Methods： The separation was performed on Diamonsil C18 column （5μm,250mm × 4.6mm） , The mobile phase consisted of acetonitrile （A） - water （B） with Linear gradient elution ; at a flow rate of 1.0 mL· min^- 1 ; the warelength of detector was 203 nm ; the column temperature was 30℃ . Result： The calibration curves showed good linearity in the range of 2.555 - 12.775μg （notoginsenoside R1 ） ,8. 125 -- 40. 625μg （ ginsenoside Rg1 ） and 7. 665 -- 38. 325μg （ ginscnoside Rbl ）. The average recoveries rate （ n = 6） were 99.2% ,99.8% and 99.5% , RSD were 0.52% ,0.28% and 0.45% .Conclusions ： The method is convenient,rapid,economical and accurate. It can be use for quality control of Xuesaitong Capusle.
出处
《内蒙古中医药》
2009年第10期116-117,共2页
Inner Mongolia Journal of Traditional Chinese Medicine
