摘要
目的:建立经真菌转化前后三七中人参皂苷Rg3的含量测定方法。方法:采用HPLC法,色谱柱为Phenomex C18(250mm×4.6mm,5μm),流动相为乙腈-水,梯度洗脱;柱温30℃,检测波长为203nm,流速1.0mL/min,进样量为20μL。结果:人参皂苷Rg3的回归方程:Y=35672013X-2413,r=0.99992,在0.0019～0.2488 mg/mL范围内有良好的线性关系,平均回收率为99.56%,RSD=1.48%(n=8)。结论:该方法简便、快捷、准确、重复性好,可用于三七发酵产物中人参皂苷Rg3的含量测定。
Objective ： To establish a assay method of ginsenoside Rg3 in Panax pseudo - ginseng before and after the fungal transformation by HPLC. Methods： A Phenomex C18 （250mm × 4.6mm, 5 μm） column served as stationary phase and acetonitrile -water in gradient elution mode as mobile phase; The column temperature was 30℃ and the detection wavelength was 203nm, the flow rate was 1.0mL/min and the injection volume was 20μL. Results： A good linearity of ginsenoside Rg3 was in the range of 0. 0019 ~ 0.2488 mg/mL and r = 0. 99992; The average recovery rate was 99.56% and RSD = 1.48% （ n = 8）. Conclusion： This method is simple, fast, accurate, reproducible and can be used for determining the content of ginsenoside Rg3 in Panax pseudo - ginseng before and after the fungal transformation.
出处
《中华中医药学刊》
CAS
2011年第9期2063-2064,共2页
Chinese Archives of Traditional Chinese Medicine
基金
上海市科委创新行动计划项目(09140903600)
上海市教委项目(10YZ51)
