摘要
目的:建立人参皂苷Rg3和其代谢产物人参皂苷Rh2血浆药物测定方法,探讨它们在大鼠体内药代动力学情况。方法:采用交叉给药设计,应用高效液相色谱法测定Wistar大鼠单次单剂量灌胃人参皂苷Rg3(50 mg/kg)后的血液中人参皂苷Rg3和人参皂苷Rh2的浓度,利用DAS2.0软件计算药动学参数。结果:口服人参皂苷Rg3后,人参皂苷Rg3在大鼠体内药-时曲线呈二室模型,主要药动学参数Cmax(81.55±24.57)mg/L,t1/2(4.27±1.35)min,AUC0-t(219.25±81.38)mg·min/L。人参皂苷Rh2在大鼠体内药-时曲线同样呈二室模型,主要药动学参数Cmax(6.17±1.34)mg/L,t1/2(3.25±0.17)min,AUC0-t(11.48±3.72)mg·min/L。结论:人参皂苷Rg3口服灌胃后,虽然在大鼠体内可以代谢为人参皂苷Rh2,但是人参皂苷Rh2在体内的量远小于人参皂苷Rg3。
Objective: To study on the pharmacokinetics of ginsenoside Rg3 and ginsenoside Rh2,which is the metabolism in rat by developing a high performance liquid chromatography(HPLC) method to determine the ginsenoside Rg3 and ginsenoside Rh2 in rat plasma after gastric administration with ginsenoside Rg3.Method: Applying to double cycle self crossover design,a single oral dose of 50 mg/kg ginsenoside Rg3 was administered to rats.The plasma concentrations of the studied components were determined by HPLC and the pharmacokinetic parameters were calculated by DAS 2.0.Result: The concentration-time curves of ginsenoside Rg3 fitted to two compartment model in rats.The Cmax(81.55±24.57) mg/L,t1 /2(4.27±1.35) min,AUC 0-t(219.25±81.38) mg·min/L,respectively.The concentrationtime curves of Ginsenoside Rh2 fitted to two compartment model in the rats.The Cmax(6.17±1.34) mg/L,t1 /2(3.25±0.17) min,AUC 0-t(11.48±3.72) mg·min/L.Conclusion: after gastric administration of ginsenoside Rg3,although ginsenoside Rg3 can be metabolized to ginsenoside Rh2,but the amount of ginsenoside Rh2 in rat plasma is much smallet than ginsenoside Rg3.
出处
《中药药理与临床》
CSCD
北大核心
2015年第2期22-25,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
辽宁省博士后基金
关键词
人参皂苷RG3
人参皂苷RH2
药动学
Ginsenoside Rg3(人参皂苷 Rg3)
Ginsenoside Rh2(人参皂苷 Rh2)
pharmacokinetics
