摘要
目的利用兔骨髓间充质干细胞联合高压氧治疗兔脊髓冲击伤,评价其治疗效果是否优于其两种治疗方法单独应用的效果。方法建立T9-T10段大白兔脊髓冲击伤模型,通过Reuter、改良Tarlov评分和体感诱发电位选择脊髓损伤程度相同的48只大白兔,随机分为4组,每组12只。12 h后对各组分别作空白对照、高压氧治疗、骨髓间充质干细胞移植、两者联合治疗方面的干预。6周后行运动感觉功能评分、免疫荧光示踪、甲苯胺蓝染色、免疫组化染色(Bax、Bcl-2检测凋亡)等检测,比较各组指标的差异。结果高压氧治疗组和骨髓间充质干细胞移植组的运动学评分及病理学指标均优于空白对照组,差异有统计学意义(P<0.05),两者联合治疗组的相关指标较其他治疗组效果明显,差异有统计学意义(P<0.05)。结论单独应用干细胞移植和高压氧治疗均能对脊髓冲击伤产生一定的修复作用,两种治疗措施联合能发挥协同作用,增强治疗效果。
Objective To therapy spinal cord injury by bone marrow mesenchymal stem cells and hyperbaric oxygen,to evaluate whether their combined treatment effect is better than their respective effect when used separately or not.Methods Established the models of T9-T10 spinal cord injuries,evaluated the extent of injuries by making kinematics score and somatosensory evoked potential score of each rabbit after 12 h,then 48 rabbits were randomly divided into four groups（n=12）,which treated nothing,bone marrow mesenchymal stem cells transplantation,hyperbaric oxygen intervention,combined treatments,respectively.Six weeks later,evaluated with kinematics score,and immunofluorescence tracing method,toluidine blue stain,immunohistochemistry（Bax,Bcl-2 detection of apoptosis）,and estimated the therapeutic efficacy of each group.Results After six weeks,we found that the treatment of combined treatment on spinal cord injury was better than the other groups significantly（P0.05）.Conclusion The therapeutic effects of bone marrow mesenchymal stem cells transplantation combined with hyperbaric oxygen on spinal cord blast injury is clear.Two therapy can play a synergistic effect and result in better treatment.
出处
《安徽医科大学学报》
CAS
北大核心
2011年第9期870-874,共5页
Acta Universitatis Medicinalis Anhui
基金
解放军105医院院管重点课题(编号:2010YG18)
关键词
骨髓
间质干细胞移植
高压氧
脊髓损伤/治疗
bone marrow
mesenchymal stem cell transplantation
hyperbaric oxygenation
spinal cord injuries/therapy
