摘要
目的比较静脉移植与局部移植骨髓间充质干细胞(Bone Marrow Stromal Cells,BMSCs)对脊髓损伤大鼠运动功能恢复的效果。方法计算机检索Medline、EMbase、The Cochrane Library(2014年第10期)、中国生物医学文献数据库、万方数据库、中国知网1990~2014年第10期间的静脉移植与局部移植BMSCs治疗脊髓损伤大鼠的随机对照实验。使用Rev Man 5.2.0软件进行Meta分析,纳入研究分为脊髓损伤后3天内细胞移植(3天组)和脊髓损伤后7天细胞移植(7天组)2个亚组,对细胞移植后2、4、6周三个时间点的BBB评分进行分析。结果共6篇文献符合纳入标准。Meta分析结果示:细胞移植后2周,3天组静脉与局部移植BBB评分差异无统计学意义(WMD:-1.15[-2.72,0.42],=0.15),7天组静脉移植BBB评分低于局部移植,差异有统计学意义(WMD:-1.02[-1.99,-0.05],<0.05);细胞移植后4周,3天组静脉与局部移植BBB评分差异无统计学意义(WMD:-0.26[-2.26,1.74],=0.80),7天组静脉移植BBB评分低于局部移植,差异有统计学意义(WMD:-0.96[-1.85,-0.07],<0.05);细胞移植后6周,3天组静脉与局部移植BBB评分差异无统计学意义(WMD:2.56[-4.39,9.51],=0.47),7天组静脉移植BBB评分低于局部移植,差异有统计学意义(WMD:-1.72[-2.71,-0.73],<0.05)。结论脊髓损伤后3天内进行BMSCs移植,局部移植和静脉移植对改善大鼠后肢运动功能没有明显区别;脊髓损伤后7天进行移植,局部移植对大鼠后肢运动功能的恢复效果优于静脉移植。
Objective To compare motor function after intralesional ,intravenous transplantation of Bone Marrow Stromal Cells （BMSCs） in rats of spinal cord injury. Methods 6 studies were searched from Medline, EMbase, CNKI, The Cochrane Library, China Biology Medicinedisc and Wanfang data-base concerning intralesional, intravenous transplantation of BMSCs in treating spinal cord injury rats between 1990 and 2014 Oct, the data of the studies were collected and Meta-analyzed by RevMan 5.2.0 software, the studies were divided into 2 groups： within 3 days after spinal cord injury to transplant BMSCs （3 day group） and 7 days after spinal cord injury to transplant BMSCs （7 day group）, and the BBB scale was meta-analyzed at 2weeks, 4weeks, 6weeks. Results 2 weeks after transplantation, no difference in BBB scale was found between intralesional and intravenous transplantation in 3 day group （WMD： - 1.15 [-2.72,0.42], P=-0.15）, the BBB scale of intralesional transplantation was higher than intravenous transplantation in 7 day group （WMD： -1.02 [- 1.99, -0.05], P〈0.05）; 4 weeks after transplantation, no difference in BBB scale was found between intralesional and intravenous transplantation in 3 day group （WMD： -0.26 [-2.26,1.74], P=-0.80）, the BBB scale ofintralesional trans- plantation was higher than intravenous transplantation in 7 day group （WMD： -0.96 [-1.85, -0.07], P〈0.05）; 6 weeks after transplantation, no difference in BBB scale was found between intralesional and intravenous transplantation in 3 day group （WMD： 2.56 [-4.39,9.51], P=0.47）, the BBB scale ofintralesional transplantation was higher than intravenous transplantation in 7 day group （WMD： -1.72 [-2.71, -0.73], P〈0.05）. Conclusion Within 3 days after spinal cord injury to transplant BMSCs no difference in motor ruction was found between intralesional and intravenous transplantation. 7 days after spinal cord injury to transplant BMSCs, the motor function of intralesional transplantation was better than intraveno
作者
高中洋
李浩鹏
秦杰
宋辉
贺西京
Gao Zhongyang Li Haopeng Qin Jie et al(Department of Orthopedics, Second Affiliated Hospital, College of Medicine, Xi＇an Jiaotong University, Xi＇an Shanxi, 710004, China)
出处
《生物骨科材料与临床研究》
CAS
2017年第1期4-9,共6页
Orthopaedic Biomechanics Materials and Clinical Study
基金
国家自然科学基金(批号:81571209)
关键词
脊髓损伤
骨髓间充质干细胞
局部移植
静脉移植
BBB评分
Spinal cord injury
Bone Marrow Stromal Cells
Intralesional transplantation
Intravenous transplantation
BBB scale
