摘要
目的探讨脑缺血后,外源性植入的骨髓基质干细胞(bone marrow stromal cells,BMSCs)在脑内的迁移和分化,及脑缺血后自体神经干细胞(neural stromal cells,NSCs)的动员情况。方法体外培养、扩增BMSCs 后,用绿色荧光染料PKH67标记BMSCs,通过静脉和脑内局部移植等途径将BMSCs植入大脑中动脉闭塞的SD大鼠脑内,10d后取材,免疫荧光检测BMSCs的分化、迁移情况;并比较假手术和脑缺血时脑内自体NSCs的数量。结果 (1)静脉移植组两侧脑半球中PKH67+细胞数分别是46．4±2．92和21．8±1．13／100mm2(P<0．05);(2)移植的BMSCs中40．31％表达神经元特异性标志;(3)在第8天时,脑缺血半球和非脑缺血半球Nestin+细胞数分别为 19．5±10．08和7±1．41,差异显著(P<0．05)。结论脑缺血可激活脑内自身的NSCs,使它们迁移至脑缺血半球并促进其修复;外源性BMSCs通过局部或静脉移植后可迁移到缺血脑组织附近,并且部分分化为神经元样细胞,表达神经元的特异性标志。
Objective To explore the migration and differentiation of exogenous bone marrow stromal cells （BMSCs） after transplanted into model rats of middle cerebral artery occlusions （MCAO） and the activation of entogenous neural stem cells （NSCs）. Methods BMSCs of SD rats were dissociated, cultured and proliferated in vitro, then the BMSCs marked with PKH67 were transplanted into permanent MCAO models of SD rats by different ways. After 10 days, the migration and differentiation of BMSCs were observed and identified by immunofluorescence method. Meanwhile the numbers of entogenous NSCs were compared in group of shamming operation and group of cerebral ischemia. Results （1）In two brain hemispheres of vein transplantation group is 46.4±2.92 and 21.8±1.13/100mm^2, respectively （P〈0.05）. （2）It is about 40.31% of transplanted BMSCs expressed neuronspecific enolase （NSE）. （3）On 8d ,The number of positive cells in the ischemic brain hemisphere is more than that in the nonischemic brain hemisphere （P〈0.05）. Conclusions Cerebral ischemia activates the NSCs in the brain which can migrate and promote the repairment of injured brain tissue. Exogenous BMSCs migrate to ischemic brain hemisphere after transplants into MCAO model rats,and some of them differentiate into neuron like cells expressing NSE.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2005年第6期523-526,T0001,共5页
Journal of Apoplexy and Nervous Diseases
基金
国家自然科学基金(30140015)
黑龙江省重大科技攻关项目(GA01C02)
关键词
骨髓基质干细胞
脑缺血
神经干细胞
细胞移植
迁移
Bone marrow stromal cells
Cerebral ischemia
Neural stem cells
Cell transplantation
Migration
