摘要
目的:探讨溴区包含蛋白7(BRD7)的亚细胞定位以及bromodomain结构域对BRD7亚细胞定位的影响。方法:首先采用GFP介导的亚细胞定位方法,在非洲绿猴肾COS7细胞中考察BRD7的亚细胞定位;然后分别构建bromodomain缺失型的BRD7重组体pCMV-Myc/BRD7△brd和pEGFP-C2/BRD7△brd,并通过GFP介导的亚细胞定位方法和间接免疫荧光方法,分别在COS7中考察bromodomain缺失型BRD7的亚细胞定位,从而探讨BRD7的bromodomain结构域对其亚细胞定位的影响。结果:成功构建了pCMV-Myc/BRD7△brd和pEGFP-C2/BRD7△brd。BRD7及bromodomain缺失型BRD7在COS7中均主要定位在细胞核,与染色质的分布存在一定程度的相似性。结论:bromodomain结构域的缺失并不影响BRD7的细胞核分布,但是在核内的分布模式发生了一定的改变。
Aim：To investigate the subcellular localization of BRD7 and the effects of bromodomain on its localization.Methods：Firstly,the subcelluar localization of BRD7 was detected in COS7 cells by direct GFP fluorescence.Secondly,bromodomain deleted BRD7 mutations,pCMV-Myc/BRD7△brd and pEGFP-C2/BRD7△brd,were constructed to investigate the effects of bromodomain on the subcellular localization of BRD7 in COS7 cells by GFP fluorescence and indirect immunofluorescence.Results：It was found that BRD7 and bromodomain deleted BRD7 mutations were all mainly localized in nucleus,which was similar to the nuclear distribution of chromatin,to some extent.Conclusion：Bromodomain deletion may not affect the nuclear localization of BRD7,but the localization pattern of BRD7 in nucleus is changed.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2011年第4期542-546,共5页
Journal of Zhengzhou University(Medical Sciences)
基金
国家自然科学基金资助项目30300175
30200135
30400238
30470367
