摘要
应用间接免疫荧光抗体法可在部分硬度病CREST综合征患者血清中发现与染色体着丝粒部分有特异反应的抗体,称抗着丝粒抗体,简称ACA.着丝粒抗原是一类蛋白质,含CENP-A(17kd)、CENP-B(80kd)及CENP-C(140kd)三种组分,只有具有功能的着丝粒才与ACA发生反应,故ACA对了解着丝粒的活性功能、形态及数目有重要意义.在肿瘤细胞及临床染色体异常中,双着丝粒染色体及各种易位的染色体很常见,应用ACA方法对其进行研究有理论和实践意义.
The discovery that individuals with CREST-syndrome scleroderma (calcino-sis cutis, Raynaud phenomenon, eosinophilia, telangiectasia) have anticentromere antibodies (ACA) in their peripheral blood has made it possible to visualize centromeres using indirect immunofluorescence staining. We have studied two tumor cells lines (k562 and SP2/0) using ACA and immunofluorescence techniques. In SP2/0 cells, two abnormal chromosomes were observed: One had a Robertsonian translocation and the other had a tandem translocation. Both were dicentric chromosomes, and the centromeres were functionally active. C-banding cannot reveal these specificities. It has been proposed that abnormal active dicentric chromosomes are mitotically unstable. This indirect immunofluorescence method may have its use in clinical cytogenetics.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1991年第6期456-458,T025,共4页
Acta Academiae Medicinae Sinicae
