摘要
目的研究艾叶不同组分单次给药致小鼠肝毒性的时毒、量毒关系。方法取小鼠按不同时间点或不同剂量分组,观察小鼠死亡情况和毒性反应,检查血清ALT、AST,计算肝、脾、胸腺脏器指数。结果艾叶水提组分和挥发油组分对小鼠单次肝毒性"量-时-毒"关系研究为:小鼠单次灌服8.0g/kg的艾叶水提组分和0.34 mL/kg的艾叶挥发油组分后,血清ALT、AST值随时间不同造成肝损害程度不同:艾叶水提组分ALT、AST均在药后2 h达到高峰,毒性持续时间约达72h。艾叶挥发油组分ALT在给药后4 h、AST在给药后6h达到高峰,毒性持续时间约72 h;均可导致肝脏指数明显升高,艾叶水提组分在8.0、5.6、3.92、2.74、1.9、1.33g/kg剂量范围内、艾叶挥发油组分在0.34、0.27、0.23、0.19、0.15、0.13 mL/kg剂量范围内,对肝组织产生明显损伤,且随剂量的增大,ALT、AST升高显著。结论小鼠单次灌服艾叶不同组分均可造成一定的急性肝损伤,其对肝损伤均呈现有一定的"量-时-毒"关系。关于其肝脏损伤的机制有待进一步研究。
Objective To study the ＂dose-time-toxicity＂ relationship of hepatotoxicity caused by single dose water extracted and volatile oil components of Folium Artemisiae Argyi to mice.Methods Mice are grouped according to different time or dose points,to observe the death condition and toxicity of mice,detect ALT、 AST in serum,calculate liver,spleen,thymus index.Results Compared with the control group,ALT,AST level in serum of water extracted components are peaked after 2 hours＇ administration,and restored near normality after 72 hours.ALT level in serum of volatile oil components are peaked after 4 hours＇ administration,while AST level in serum of volatile oil components are peaked after 6 hours＇ administration,and they all restored near normality after 72 hours.The water extracted components of（8.0~1.33） g·kg-1 and volatile oil components of（0.34~0.13） mL·kg-1 have obvious damage to the liverorganization,and ALT,AST in serum increased significantly with the dose increasing.Conclusion The water extracted and volatile oil components gavaged certain dose one time to mice could cause obvious acute hepa-injury,and there were certain time-toxicity and dose-toxicity relationships.
出处
《中国药物警戒》
2011年第7期392-396,共5页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题(2008GG2NS02021)
关键词
艾叶
不同组分
单次给药
小鼠
肝毒性
Folium Artemisiae Argyi
water extracted and volatile oil components
mice
hepatotoxicity
＂dose-time-toxicity＂ relationship
