摘要
目的研究艾叶不同组分多次给药致小鼠肝毒性的"量-时-毒"关系。方法取小鼠按不同时间点或不同剂量分组,观察小鼠死亡情况和毒性反应,分别于给药后相应时间点检查血清肝功指标和肝脏组织ALT、AST活性,计算肝、肾脏器指数。结果艾叶水提组分和挥发油组分对小鼠多次给药肝毒性"量-时-毒"关系研究为:ALT、AST在给药后1天即有明显升高3,天肝毒性明显,可持续到7天。与正常组比较,给药后7天之内,水提组分在(1.17～9.0)g.kg-1剂量范围、挥发油在(0.13～0.25)mL.kg-1剂量范围之内,艾叶水提组分和挥发油均可造成明显的肝毒性损伤,表现ALT、AST、AKP、TBI升高,ALB降低,肝体比值增高:呈现明显的量效和时效关系;肝毒性作用程度挥发油>水提组分。结论多次给小鼠一定剂量的艾叶水提组分和挥发油组分均可造成明显的肝损伤,并呈现明显的肝毒性"量-时-毒"关系。
Objective To study the ＂dosage-time-toxicity＂ relationship of hepatotoxicity induced by multiple intragastric administration of water extracted and volatile oil components of Folium Artemisiae Argyi to mice.Methods Mice are grouped according to different time or dose points,to observe the death condition and toxicity of mice.The changes of the activity of ALT,AST and liver,kidney index were detected.Results On the first day after administration the hepatotoxicity which displayed with obvious increase of activity in serum ALT,AST and hepar index appeared.On the 3th day the hepatotoxicity kept a higher level that the active units in serum ALT,AST were significantly higher than the normal group.On the 7th day after administration ALT,AST level in serum are restored near normality.Compared with the normal group,within 7 days after the administration,water extracted components in（1.14～9.0）g·kg-1 doses of scope,volatile oil components in（0.13～0.25）ml·kg-1 doses of scope,they all cause significant damage to liver,the activity of ALT,AST,AKP,TBI elevated,while ALB reduced,and liver ratio increased,it shows there were obvious ＂dosage-time-toxicity＂ relations,Hepatotoxic effects volatile oil components water extracted components.Conclusion Multiple intragastric administrations of water extracted and volatile oil components of Folium Artemisiae Argyi with certain dosage may induce acute hepatotoxical injury in mice and show an obvious＂dosage-time-toxicity＂ relationship.
出处
《中国药物警戒》
2011年第7期397-400,共4页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题(2008GG2NS02021)
关键词
艾叶
不同组分
多次给药
小鼠
肝毒性
Folium Artemisiae Argyi
water extracted and volatile oil components
＂dosage-time-toxicity＂relationship
