摘要
目的研究山豆根不同组分多次给药对小鼠肝毒性损伤的作用程度。方法连续7天给小鼠灌胃不同剂量的山豆根水提、醇提组分,观察小鼠一般状况,于给药后第7天检测小鼠血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、碱性磷酸酶(AKP)的活性和总胆红素(TBI)、白蛋白(ALB)的含量,计算肝脏指数,并观察肝组织形态变化。结果山豆根水提、醇提组分高、中剂量组在药后第7天可致小鼠体重增长缓慢,血清中ALT、AST、AKP活性升高,TBI含量升高,ALB含量下降,肝脏指数增大,并出现肝细胞病理组织学变化,水提组分对上述指标的影响比醇提组分明显。结论多次给小鼠高、中剂量的山豆根水提组分和醇提组分均可造成明显的肝损伤,既可致肝功能指标的改变,又可致肝细胞器质性病变,且水提组分的肝毒性大于醇提组分。
Objective To investigate the hepatotoxic effect induced by different components of Radix et Rhizoma Sophorae Tonkinensise（RRST） in mice. Methods Mice are divided into different groups such as normal, water extract and alcohol extract group to observe the normal conditions after seven days administration. The activity of ALT、 AST、AKP、TBI、ALB in serum, the liver weight and volume ratio, the changes of liver morphology were observed on the 7th day after administration. Results The mice＇s body weight was effected after given water extract and alcohol extract of RRST, both the ALT、AST、AKP and TBI were increased, while the ALB was decreased, the liver weight and volume ratio were increased and the liver pathology was changed, the water extract group has more obvious influence. Conclusion Multiple intragastric administrations of water extract and alcohol extract of RRST with certain dosage may induce acute hepatotoxic injury in mice especially the water extract, it causes not only the changes of liver function index, but also the structural pathological changes of hepatic cell.
出处
《中国药物警戒》
2011年第10期577-580,共4页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题(2008GG2NS02021)
关键词
山豆根
不同组分
肝毒性
Radix et Rhizoma Sophorae Tonkinensise
hanges of hepatic cell water extract and alcohol extract
hepatotoxicity
