摘要
采用硅胶柱层析法分离绞股蓝总皂苷,以氯仿-甲醇混合液为流动相进行梯度洗脱,薄层层析法跟踪检测。5 000 g绞股蓝干草经乙醇提、石油醚脱脂、正丁醇萃取、AB-8大孔吸附树脂脱糖、D-296阴离子交换树脂脱色后得到总皂苷7.87 g。5 g绞股蓝总皂苷进行硅胶柱层析分离,在体积比为9.5∶0.5和9∶1的氯仿-甲醇洗脱液洗脱下得到3种较纯的绞股蓝皂苷的单体组分,得率分别为2.6%、3.0%和5.2%。经高效液相色谱检测其纯度,组分A、B、C纯度分别为88.73%、93.11%和78.54%。3种皂苷较粗品纯度得到很大提高,且洗脱液比例和体积的确定为大量制备这3种绞股蓝单体皂苷提供了理论依据。
The total gypenoside was separated and purified by silica gel column. Chloroform and methanol were used as mobile phase in gradient elution, the sample was determined by TLC. 7.87 g total saponin was obtained from 5 000 g Gynostemma pentaphyllum by the process of extracted with alcohol, defatted with ligarine, leached with normal butanol, desugared with AB-8 macro porous resin, decolored with D-296 anion ion exchange resin. Three kinds of gypenoside monomer were obtained from 5 g total gypenoside by the proportion in 9.5 ＂ 0.5 and 9 ~ 1 chloroform and methanol as mobile phase. The yields were 2.6Y00, 3.0%, 5.2%and the purity was up to 88.73%, 93.11%, 78.54% respectively. The result indicated that the silica gel column is a good separation function to gypenoside.
出处
《大连工业大学学报》
CAS
北大核心
2011年第3期173-176,共4页
Journal of Dalian Polytechnic University
基金
国家自然科学基金资助项目(30371744)
辽宁省科学技术基金资助项目(20042132)
辽宁省教育厅创新团队项目(2009T007)
