摘要
目的:研究蒺藜皂苷(STT)对大鼠小肠α-葡萄糖苷酶的抑制作用及对餐后血糖水平的影响。方法:提取大鼠小肠α-葡萄糖苷酶,测定STT对其活性的抑制作用;一次性灌胃蔗糖(2 g.kg-1)或葡萄糖(2 g.kg-1),同时灌胃STT(100 mg.kg-1),测定60 min后血糖水平的变化;每天一次性灌胃STT(100 mg.kg-1),连续14 d,测定小肠α-葡萄糖苷酶活性以及灌胃蔗糖(2 g.kg-1)60 min后血糖水平的变化。结果:0.1,1,10 mg.mL-1的STT能够显著抑制α-葡萄糖苷酶的体外活性(P<0.001),抑制率分别为(20.83±1.66)%,(43.73±2.39)%和(52.62±2.69)%;STT显著抑制大鼠一次性灌胃蔗糖60 min后血糖水平的升高(P<0.01),变化幅度为对照的(52.61±6.24)%,但对灌胃葡萄糖没有作用;连续灌胃STT 14 d后,α-葡萄糖苷酶活性显著低于对照(P<0.05),为对照的(58.17±3.24)%,灌胃蔗糖60 min后血糖变化幅度为对照的(69.50±4.28)%(P<0.05)。结论:STT抑制大鼠餐后血糖水平的升高,是由其对小肠α-葡萄糖苷酶的抑制作用实现的。
Objective： To determine the effects of saponins from Tribulus terrestris （SIT） on small intestinal α-glucosidase and postprandial blood glucose levels in rats. Method： The inhibitory effects of STT on α-glucosidase extracted from small intestines in rats were carried out in vitro. The blood glucose levels were measured after 60 rain when sucrose （2 g·kg^-1） or glucose （2 g·kg^-1） was administered orally with STT（100 mg·kg^-1）. After treated with STT （100 mg·kg^-1） for 14 d, the activities of α-glucosidase were determined daily, as well as the postprandial blood glucose levels after oraly administered sucrose （2 g·kg^-1）. Result： STT at concentrations 0.1, 1 and 10 mg·kg^-1 reduced significantly the activities of α-glucosidase with inhibitory rates of （20.83 ± 1.66 ） %, （ 43.73 ± 2.39 ） % and （ 52.62 ± 2.69 ） %, respectively. In facts STT （100 mg·kg^-1） considerably decreased the blood glucose levels which was 52.61% of that of the control in rats co- administered orally with sucrose （2 g·kg^-1）. Hrwever, it showed no such effect on the rats co-administered orally with glucose （2 g·kg^-1）. After orally administered of STT for 14 d, the activity of α-glucosidase was significantly reduced （ P 〈 0.05） to （58.17 ± 3.24） % of that those in control. Meanwhile, The rats were oral administered with sucrose, the increase of postprandial blood glucose levels were （ 69.50 ± 4.28） % of that in control 60 min later （ P 〈 0.05）. Conclusion： It was through inhibiting the activity of α-glucosidase in small intestines that STT significantly retarded the increase in postprandial blood glucose levels in rats.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第11期910-913,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30370158)
