摘要
目的研究叶黄素、大豆甙元、卵磷脂对小鼠认知行为的影响。方法各组经灌胃持续给药6周,采用跳台和Morris水迷宫对小鼠进行行为学测试,取小鼠血清及脑组织进行生化检测。结果大豆甙元组、卵磷脂组、混合组脑丙二醛(MDA)含量分别为(3.4±0.4)、(3.4±0.4)、(3.2±0.3)nmol/(mg.prot);明显低于空白对照组的(3.8±0.5)nmol/(mg.prot)(P<0.05,P<0.01);叶黄素组、大豆甙元组、混合组及阳性对照组Morris逃避潜伏期分别为(23.09±6.59)、(22.29±7.33)、(21.45±5.45)、(17.54±6.65)s,明显低于空白对照组的(45.80±9.70)s(P<0.05,P<0.05);叶黄素组、大豆甙元组、卵磷脂组、混合组脑5-羟色胺(5-HT)含量分别为(210.08±20.46)、(222.95±15.87)、(225.18±24.56)、(237.97±29.30)ng/mL,明显高于空白对照组的(171.61±32.94)ng/mL(P<0.01),叶黄素、卵磷脂、混合组脑去甲肾上腺素(NE)含量分别为(104.09±7.63)、(106.12±7.25)、(108.84±16.94)ng/mL,明显高于空白对照组的(93.25±7.33)ng/mL,差异均有统计学意义(P<0.05,P<0.01)。结论单独及联合补充对小鼠认知行为能力有不同程度改善作用。
Objective To study the effects of lutein,daidzein,and lecithin on cognitive behavior in mice.Methods The lutein,diadzein,lectithin and their combinations were administered to male Kunming mice with gavave for six weeks.Then step down test and Morris water maze test were conducted.The biochemical indices for serum and brain tissue of the mice were measured.Results The contents of malondialdehyde in the brain of diadzein group（3.4±0.4 nmol/mg·prot）,lecithin group（3.4±0.4 nmol/mg·prot）,and combination group（3.2±0.3 nmol/mg·prot） were significantly lower than that of blank control group（3.8±0.5 nmol/mg·prot,P0.05 or P0.01）.The latency periods of Morris water maze test for lutein group（23.09±6.59s）,daidzein group（22.29±7.33s）,and combination group（21.45±5.45s） were significantly lower than that of the control group（45.80±9.70 s,P0.05 or P0.01）.The contents of 5-hydroxytryptamine in the brain of lutein group（210.08±20.46 ng/ml）,daidzein group（222.95±15.87 ng/ml）,lecithin group（225.18±24.56 ng/ml）,and combination group（237.97±29.30 ng/ml） were significantly higher than that of the control group（171.61±32.94 ng/ml,P0.01 for all）.The contents of norepinephrine in the brain of lutein group（101.22±6.29 ng/ml）,lecithin group（106.12±7.25 ng/ml）,and combination group（108.84±16.94 ng/ml） were significantly higher than that of the control group（93.25±7.33 ng/ml,P0.01 or P0.05）.Conclusion The cognitive behavior could be ameliorated in different extent by oral administration of lutein,daidzein,lecithin lonely or combined in mice.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第5期600-602,共3页
Chinese Journal of Public Health
基金
浙江省卫生高层次创新人才培养项目
浙江省"151"人才工程第二层次培养项目
关键词
叶黄素
大豆甙元
卵磷脂
认知行为
联合协同
lutein
daidzein
lecithin
cognitive behavior
combination and cooperation
