摘要
基于已经建立的藏红花细胞悬浮培养体系,研究了其悬浮培养动力学。结果表明,悬浮培养时细胞的生长周期约为20 d,在20 d时生物量达到最大,为12.3 g DW/L,但是藏红花素的合成周期大约为28 d,在28 d时藏红花素的含量和产量达到最大,分别为95.8 mg/g和0.92 g/L。藏红花素的积累与细胞的生长之间的关系为半生长偶联型,为其生物反应器放大培养提供了参数等理论依据。
Based on the established cell suspension culture system of Crocus sativus L.,its kinetics was investigated.The highest biomass reached 12.3 g DW/L on 20th d,and the highest crocin content and production were obtained on 28th d,both of which were 95.8 mg/g and 0.92 g/L,respectively.The process of croin biosynthesis was semi-associated with the cell growth of Crocus sativus L..This research could provide references for the scale-up culture of Crocus sativus L.cell by bioreactor.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第4期102-105,共4页
Biotechnology Bulletin
基金
中国科学院"西部行动高新技术项目"(KGCX-YW-509)
关键词
藏红花
悬浮
培养体系
动力学
生物反应器
Crocus sativus L.Suspension Culture system Kinetics Bioreactor
