摘要
昆虫通过降低靶标酶敏感性及增强对杀虫剂的代谢能力产生抗药性。蝇毒磷是防治柞蚕饰腹寄蝇(Blepharipa tibia-lis Chao)的常用有机磷杀虫剂,为探讨柞蚕饰腹寄蝇不同地理种群对蝇毒磷的抗药性分化程度,分析来自辽宁省瓦房店市、凤城市、庄河市、开原市和西丰县的5个柞蚕饰腹寄蝇地理种群体内的乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GST)、细胞色素P450(CYP450)和羧酸酯酶(CarE)4种酶的活性及蝇毒磷对这些酶活性的体外抑制率。结果表明:来自瓦房店市和凤城市的柞蚕饰腹寄蝇地理种群的AChE比活力及蝇毒磷对酶活性的体外抑制率均显著高于其它3个地理种群;瓦房店市地理种群的GST比活力显著高于其它4个地理种群,蝇毒磷对酶活性的体外抑制率显著高于除庄河市地理种群外的其它3个地理种群;庄河市地理种群的CYP450比活力显著高于来自瓦房店市、凤城市和西丰县的3个地理种群,但与开原市地理种群相比差异不显著,蝇毒磷对瓦房店市地理种群的CYP450活性的体外抑制率显著高于其它4个地理种群;瓦房店市与凤城市地理种群的CarE比活力均显著高于来自庄河市、开原市和西丰县的3个地理种群;蝇毒磷对瓦房店市和凤城市地理种群的CarE活性的体外抑制率显著高于庄河市地理种群,对其它地理种群间的CarE活性的体外抑制率差异不显著。根据以上检测结果,推测来自庄河市的柞蚕饰腹寄蝇地理种群对蝇毒磷的抗性相对较强,而来自瓦房店市的柞蚕饰腹寄蝇地理种群对蝇毒磷的抗性相对较弱。
Insect develop pesticide resistance by reducing the sensitivity of target enzyme and/or enhancing the metabolism of pesticide.Coumaphos is a common organophosphorus pesticide for Blepharipa tibialis control.To investigate the resistance differentiation degrees of Blepharipa tibialis＇s different geographical populations to coumaphos,we analyzed the activity of four enzymes,namely acetylcholinesterase（AChE）,glutathione S-transferase（GST）,cytochrome P450（CYP450） and carboxylesterase（CarE） in Blepharipa tibialis of five geographical populations from Wafangdian,Fengcheng,Zhuanghe population,Kaiyuan and Xifeng in Liaoning Province,respectively.Besides,the in vitro inhibition rates of coumaphos to these four enzymes were investiga-ted.The results showed that the specific activities of AChE and in vitro inhibition rate of coumaphos to this enzyme in Wafangdian population and Fengcheng population were significantly higher than those in other three geographical populations.The specific activity of GST in Wafangdian population was significantly higher than those in other four geographical populations,and the in vitro inhibition rate of coumaphos to GST was significantly higher than those of the other three geographical populations excluding Zhuanghe population.The specific activity of CYP450 in Zhuanghe population was significantly higher than those of Wafangdian,Fengcheng and Xifeng,but was not significant with that of Kaiyuan population.The in vitro inhibition rate of CYP450 in Wafangdian population was significantly higher than those of the other four geographical populations.The specific activities of CarE in Wafangdian population and Fengcheng population were significantly higher than those in Zhuanghe,Kaiyuan and Xifeng populations.The in vitro inhibition rates of coumaphos to CarE in Wafangdian population and Fengcheng population were significantly higher than that in Zhuanghe population,and were not significant with those of other geographical populations.According to the above results,we presumed
出处
《蚕业科学》
CAS
CSCD
北大核心
2011年第2期337-342,共6页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系建设专项(蚕桑)
