摘要
目的:观察慢性牙周炎牙龈组织中的凋亡细胞,并探讨其发生凋亡的机制。方法:采用透射电镜法观察牙龈组织凋亡细胞的超微结构、利用免疫组化方法检测20例慢性牙周炎患者及20例牙周健康者牙龈组织中凋亡蛋白Casepase-9、Cyt-C的表达并进行统计学分析。结果:慢性牙周炎患者牙龈组织中可观察到凋亡细胞,Case-pase-9、Cyt-C的阳性表达显著高于牙周健康组(P<0.05)。结论:慢性牙周炎较牙周健康者龈组织发生明显的细胞凋亡现象,并依赖于线粒体途径。
Objective： To observe the ultrastructure of apoptosis cell on gingival tissues of chronic periodontitis and explore the possible mechanisms of apoptosis.Methods： Under transmission electron microscopy（TEM）,the ultrastructure of apoptosis cell was observed.Immunohistochemistry was also used to investigate the expression of Casepase-9 and Cyt-C in gingival tissues of 20 patients with chronic periodontitis and 20 healthy individuals.Results： Apoptosis cells were observed in gingival tissues of patients with chronic periodontitis.The positive expression of Casepase-9 and Cyt-C in chronic periodontitis group was significantly higher than that of healthy group（P0.05）.Conclusion： Apoptosis exists in gingival tissues of chronic periodontitis,which is dependent on mitochondrial pathway.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2011年第1期52-54,共3页
Journal of Oral Science Research
