摘要
目的 观察多效生长因子（PTN）在结直肠癌中表达.方法 选取我院接受治疗的83例结直肠癌患者设为观察组,选取同期到我院进行健康检查的志愿者54例设为对照组.采用原位杂交和免疫组织化学染色法检测结直肠癌组织和正常结直肠组织中PTN mRNA及其蛋白的表达,并分析其余结直肠癌患者临床病理特征的关系.结果 观察组患者的PTN mRNA及其蛋白的表达阳性率分别为63.9％和60.2％,对照组PTN mRNA及其蛋白的表达阳性率分别为40.7％和33.3％,观察组显著高于对照组,两组间差异有统计学意义（P＜0.05）;观察组患者中PTN mRNA及蛋白的表达与年龄、性别、肿瘤类型均无明显相关（P＞0.05）,而与分化程度、TNM分期明显相关（P＜0.05）.而Ⅲ～Ⅳ期患者的PTN mRNA及蛋白的表达阳性率均高于Ⅰ～Ⅱ期患者,而高分化者均低于低分化者（P＜0.05）.结论 PTN mRNA及其蛋白在结直肠癌组织中高表达,其可能参与了结直肠癌的浸润与转移.
Objective To investigate the pleiotropic growth factor （PTN） expression in colorectal cancer.Methods The hospital treated 83 cases of colorectal cancer patients to observation group,choose the same period to our hospital for a health check to volunteer 54 cases in the control group.The expression in situ hybridization and immunohistochemical staining to detect colorectal cancer tissues and normal rectal tissue PTN mRNA and protein,and analyze the relationship between clinical and pathological features of the rest of colorectal cancer.Results The expression rate of patients in the observation group and protein PTN mRNA 63.9% and 60.2%,respectively,PTN mRNA and protein expression rate of the control group of volunteers was 40.7% and 33.3%,respectively,the PTN mRNA and proten expression rate of patients in the observation group was significantly higher than the control group of volunteers,the difference was statistically significant （P 〈 0.05） ; and the degree of differentiation observed in patients PTN mRNA and protein expression,TNM stage was significantly correlated （P 〈 0.05）.And Ⅲ-Ⅳ stage patients PTN mRNA and protein expression were significantly higher than Ⅰ-Ⅱ patients,while well-caught less than poorly differentiated （P 〈 0.05）.Conclusion PTN mRNA its protein is highly expressed in colorectal cancer tissues,which may be involved in invasion and metastasis of colorectal cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2015年第4期732-734,共3页
Chinese Journal of Experimental Surgery
关键词
多效生长因子
结直肠癌
原位杂交
免疫组织化学染色
Pleiotropic growth factor
Colorectal cancer
In situ hybridization
Immunohistochemistry
