摘要
目的:建立基于肠吸收技术以快速发现白芍吸收成分群的方法,为选择白芍质量控制指标提供参考。方法:采用大鼠肠外翻模型,收集3个质量浓度白芍提取物给药后不同时间的肠囊液样品,用HPLC进行检测分析,并计算其有效成分芍药内酯苷、芍药苷的累计吸收量。结果:白芍中5种主要成分均可进入肠囊,在45 min后能全部检测到;不同质量浓度白芍中芍药内酯苷、芍药苷在各肠段均为线性吸收,R2均大于0.9,符合零级吸收速率;芍药内酯苷和芍药苷在空肠、回肠中的吸收速率常数(Ka)随着白芍提取物浓度增加而增加(P<0.05),符合被动吸收。结论:基于肠吸收的质量评价指标选择方法(SEMAC)可以用于白芍吸收成分群的研究。肠囊对药物成分吸收有选择性,回肠与空肠相比可更多、更快地给出吸收成分信息,选择肠外翻60 min的样品检测白芍吸收成分比较适宜。
Objective ： To establish a method for quick finding of the absorption ingredients of Paeoniae Radix Alba in order to select the index of quality control. Method： The absorption ingredients of three concentration of Paeoniae Radix Alba were investigated with the in vitro-evened intestinal sac （VEIS） model. The intestinal sac fluids of jejunum and ileum were collected in different time and detected by HPLC. The accumulative absorption quantity of albiflorin and paeouiflorin were calculated, respectively. Result： Five ingredients could be detected. In different concentrations of Paconiae Radix Alba, albiflorin and paeoniflorin in various intestinal sections were the linear absorption （ R2 〉 0. 9 ）, conformed to the zero order absorption rate. The values of Ka in the jejunum and ileum were increased along with the raised dosage of the Paeoniae Radix Alba （ P 〈 0.05 ）, indicating a passive absorption manner. Conclusion： SEMAC could be used as a tool to find the absorption ingredients of Paeoniae Radix Alba. Compared with the jejunum, the ileum could provide the more absorption information. It was showed that the optimal detecting time was 60 min.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第6期687-690,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2009ZX09308-003
2009ZX09301-005
2009ZX09502-021)
中医药行业科技专业资助项目(200707009)
关键词
白芍
肠外翻技术
HPLC
吸收成分
Paeoniae Radix Alba
selection of evaluation markers by absorption components （SEMAC）
HPLC
absorption in-gredients
