摘要
以分子量100D和500D规格的透析袋做工具,对吴茱萸和吴茱萸汤水提醇沉部分进行透析纯化,分别得于<100D,100～500D以及>500D共3部分。利用高效液相色谱对不同时间透析处理过的部分化学成分进行分析,确定透析效率最佳时间点,并以此对血管活性进行体外实验。通过比较吴茱萸碱、异鼠李素和柠檬苦素3种成分在不同透析时间处理下100～500D区间内的含量,初步确定48h透析处理为最佳透析效率时间点。通过检测该时间吴茱萸汤处理组中6-姜酚、人参皂苷Re、人参皂苷Rg1和人参皂苷Rb1的含量,进一步验证48h为最佳处理时间。吴茱萸汤和吴茱萸>100D透析部分具有明显的缩血管活性,<100D透析部分对血管收缩无明显影响。表明利用不同孔径和透析袋对提取物进行样品前处理,并用于体外实验具有一定的可行性。
Dialysis tubes （Mw 100 D and Mw 500 D） were used to dialyze and purify the water extraction of Wuzhuyu （Fructus Evodiae） and Wuzhuyu decoction, and three portions of 〈100 D, 100-500 D and 〉500 D were obtained. To ascertain the peak efficiency timepoint, several chemicals treated for different time periods by HPLC were analyzed, and the activity of blood vessels was studied in vitro. By comparing different dialyzing time for three chemicals including evodiamine, isorhamnetin and limonin in 100-500D,the best treatment time was found to be 48 h. The portion of greater than 100 D significantly contracted blood vessels, while the portion of less than 100 D did not. In conclusion, it may be feasible to dispose samples using dialysis tubes with different apertures and apply the extract to pharmacological evaluation in vitro.
出处
《世界科学技术-中医药现代化》
2010年第4期515-520,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科学技术部"十一五"国家科技支撑计划项目(2006BAI08B03-09):中药复方质量控制与评价方法研究--吴茱萸汤
负责人:杜力军
中国博士后科学基金(20080440418):吴茱萸汤与吴茱萸对血管及胃肠平滑肌作用的比较研究
负责人:王秀坤
国家自然科学基金(30973896):从黄芩苷对TLRs-NOD2模式识别及相关信号转导通路的调控探讨肺
脑相关炎性病变的共有机制
负责人:邢东明
国家自然科学基金(30801523):石榴叶总酚成分对脑缺血过程中PPARγ转录活性的影响及其脑保护作用研究
负责人:雷帆
关键词
吴茱萸汤
吴莱萸
透析
高效液相
Wuzhuyu decoction, Fructus Evodiae, Dialysis tube, HPLC
