摘要
为研究脉冲电场的生物医学效应机理,基于多层介质电场模型建立了球形单细胞的场-路复合模型,提出了内外膜跨膜电位与外加电场之间传递函数的计算方法,分别分析了脉冲电场作用下内外膜跨膜电位的时域特性与频率特性。时域分析表明,不同的脉宽导致外加电场对内外膜具有不同的选择性作用。频域分析表明,细胞内外膜分别具有带通和低通滤波性能,在不同脉宽的脉冲电场(具有不同的频谱分布)作用下,将诱导出不同的生物医学效应。理论分析与医学实验结果均表明,场-路复合模型与电场模型和电路模型均吻合得很好,对电穿孔、凋亡等生物医学效应的诱导机理给出了初步统一的解释,为脉冲电场用于肿瘤治疗的机理研究和参数的合理选择提供了理论依据。
In order to research the biomedical mechanism of pulsed electric field（PEF）,the equivalent dielectric-circuit compound model of spherical biological cell and the method for calculating the transfer functions of inner and outer membranes are presented in this paper.The time-domain solution of transmembrane potential induced by time-varying electric field is introduced,and the relationship between electric field parameters and transmembrane potential is also analyzed.It is found that different duration can result in different selective effect on inner and outer membranes.Frequency-domain analysis shows that inner and outer membranes exposed to PEF exhibit band-pass and low-pass filter characteristic,respectively.Therefore,different biomedical effects will be induced in response to different field.Both academic analyses and medical experiment results show that the dielectric-circuit compound model agrees well with the dielectric model and the circuit model,and can give explanation for the electroporation and apoptosis induction.The dielectric-circuit compound model provides theoretical guidance for parameter selection and mechanism study on application of PEF in tumor treatment.
出处
《电工技术学报》
EI
CSCD
北大核心
2011年第2期14-20,33,共8页
Transactions of China Electrotechnical Society
基金
国家自然科学基金(50637020)
教育部新世纪优秀人才支持计划(NCET-06-0768)资助项目
关键词
场-路复合模型
细胞内外膜
脉冲电场
跨膜电位
时域分析
频域分析
Dielectric-circuit compound model
cellular inner and outer membranes
pulsed electric field（PEF）
transmembrane potential
time-domain analysis
frequency-domain analysis
