摘要
试验对液体深层发酵产羊肚菌菌丝体胞内多糖进行提取和分离。利用高压脉冲电场法对多糖提取过程中,不产生热量,不会对多糖结构造成破坏。利用响应面法,对电场强度、脉冲数和液料比值进行考察,得到最佳多糖提取工艺为:电场强度18kV/cm、脉冲数7和液料比值27 mL/g,此时的多糖得率达到最大值,为56.03μg/mL。采用DEAE-52纤维素离子交换柱对得到的粗多糖进行分离纯化,得到一种非极性多糖E1。进一步采用葡聚糖凝胶色谱纯化,得到一种分子量高达2.483 7×106 Da的多糖F1,约占E1的71.952%,是羊肚菌的主要成分。其功能和结构特点有待进步一研究。
The extraction and purification of endo-cellular Morchella mycelia polysaccharides （EMP） from submerged fermentation were studied. Pulsed electric field （PEF） method could lessen thermal degradation in the process of polysaccharide extraction. The optimal extraction prossessing conditions were obtained by the response surface method. The optimal conditions were as follows： electric field intensity of 18 kV/cm, pulse number of 7 and liquid-solid ratio of 27 mL/ g. The yield of polysaccharide was reached 56.03 μg/mL. The seperation and purification of polysaccharides were carried on by DEAE-52 cellulose ion exchange column and gel permeation chromatography （GPC）. One kind of macro-molecular polysaccharide named F1 with a molecular weight of 2.483 7× 10^6 Da was got. It was dominant sector in EMP accounting about 71.952%. The function and structure characteristics ofF1 needed to be researched further.
出处
《食品工业》
CAS
北大核心
2016年第12期54-58,共5页
The Food Industry
基金
2014年吉林省科技厅自然基金项目"液体发酵羊肚菌菌丝体多糖分离纯化和主要生物活性研究"(编号:20140101036JC)
2015年吉林省大学生科技创新项目"液体发酵产羊肚菌菌丝体胞内多糖纯化工艺研究及结构分析"(编号:吉农院合字[2015]第034号)
关键词
羊肚菌
胞内多糖
高压脉冲电场
提取
分离纯化
Morchella esculenta
endo-cellular polysaccharide
pulsed electric field
extraction
seperation and purification
