摘要
为确定脉冲电场生物学效应的电场参数,从细胞多层介电模型出发,通过研究其等效电路模型在外加电场中生物响应的仿真分析得出植物细胞外膜具有低通滤波特性,内膜具有带通滤波特性。同时对细胞外膜跨膜电压进行时域分析表明频率小于2.83 Hz、电场强度大于69.7 kV/m、ms/μs级脉宽的极低频脉冲电场可以在植物细胞外膜产生可逆电穿孔。采用电场强度为100 kV/m、频率为1 Hz、脉宽为80 ms的极低频脉冲电场处理萌发绿豆种子,在萌发第4 d时,绿豆种子的芽长和根长分别比对照组增加了65.8%和80.2%。
In order to determine the electric parameters of pulsed electric field（PEF） biological effects, we researched the response of the equivalent circuit model in the PEF based on multi-shelled dielectric model of spherical cell.The simulation of the transfer function revealed that the cell outer membrane had low-pass filter characteristic and the inner membrane had band-pass filter characteristic.Time domain characteristic of transmembrane potentials on cell outer membrane showed that the pulsed electric field, which had less than 2.83 Hz of PEF frequency,more than 69.7 kV/m of PEF electric field intensity,and ms/μs class of pulse width,could produce reversible electroporation on cell outer membrane.Extremely low frequency pulsed electric field of 100 kV/m,1 Hz and 80 ms was used to treat germinating mung beans seedling.It was found that the seed germination process was significantly promoted： on the 4th day of germination,and the shoot length and root length of germinating mung beans seed increased by 65.8% and 80.2% respectively compared with those of the control group.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2014年第4期253-258,283,共7页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家自然科学基金资助项目(11005136)
中科院西部之光人才培养计划资助项目(Y107130XBB)
校高层次人才基金资助项目(2012BS036
2013BS063)
关键词
脉冲电场
生物学
细胞多层介电模型
仿真
Palused electric field
Biological
Cell multi-shelled dielectric model
Simulation
