摘要
目的:研究用阳离子树脂富集纯化百部总生物碱的工艺条件。方法:以总生物碱的吸附量和解吸率为考察指标,采用UV分光光度法测定百部总生物碱的含量,对7种不同型号的阳离子树脂进行评价,并考察最佳工艺参数。结果:最佳提取工艺是:百部粉碎成粗粉,加入8倍量的90%乙醇,加热回流提取3次,每次1h。D004型阳离子交换树脂对百部总生物碱最大吸附量为0.5003mg/g,洗脱率68.45%,总生物碱转移率为58.70%,最终产品的生物碱纯度达到70%。结论:D004型树脂可用于纯化百部总生物碱,该方法简便可行。
Objective ： In order to obtain the optimal conditions for separating the alkaloids from the extract of Stemona japonica by selecting appropriate cation exchange resins. Methods ： Seven types of cation exchange resins were evaluated in separating efficiency with measuring the adsorption ratio and eluting ratio of total alkaloids as indices, and the content of total alkaloids from Stemona japonica was determined as an index by speetrophotometry to choose the optimal technological parameters. Results：The optimal result of extraction was obtained as Stemona japonica shattered into thick powder, adding eight times amount of 90% alcohol and refluxing and extracting for 3 h（ totally extracting for 3 times）. Each gram of D004 cation exchange resin could absorb 0. 5003mg of the total alkaloid, and the de- sorption ratio was 68.45%. The transfer rate of total alkaloids was 58.70%. the product purity of alkaloids was up to 70%. Conclusion：The D004 cation exchange resin can be used for purificating total alkaloids from Stemona japonica and the established procedure is simple and feasible.
出处
《中药材》
CAS
CSCD
北大核心
2011年第1期126-129,共4页
Journal of Chinese Medicinal Materials
基金
国家科技重大专项(2009ZX09313-008)
上海市创新团队及重点学科(J50302)
关键词
百部
总生物碱
纯化
Sternona japonica （ Bl. ） Miq. Lour.
Total alkaloids
Purification
