摘要
目的优选宽筋藤总生物碱提取的最佳工艺。方法采用正交试验方法考察乙醇浓度、提取次数、提取时间和溶剂用量对宽筋藤中总生物碱提取量的影响，优选宽筋藤总生物碱最佳提取工艺。结果优选得到的宽筋藤总生物碱最佳提取工艺为：盐酸-70％乙醇（1：100）混合溶液提取3次，1h／次，溶剂用量为8倍量。结论所建立方法准确可靠，重现性好，具有良好的可行性。
Objective To optimize the extraction of total alkaloids in Tinospora sinensis. Methods The orthogonal test was designed to evaluate the effect of ethanol concentration, number of times, extraction time and solvent volume on the extraction amount of total alkaloid. Results The optimum extraction process included extracting hydrochloric acid-70% ethanol （1：100） 3 times by 1 hour each time which made the amount of it 8 times more than before. Conclusions This optimized technology could be accurate, reproducible, and feasibile.
作者
郭凤霞
韩丹
刘斌
Guo Fengxia Han Dan Liu Bin.(School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China)
出处
《国际中医中药杂志》
2016年第11期1014-1016,共3页
International Journal of Traditional Chinese Medicine
关键词
宽筋藤
生物碱类
正交试验
提取工艺
Tinospora sinensis
Alkaloids
Orthogonal test
Extraction technology
