摘要
目的建立宽筋藤药材中总生物碱的含量测定方法。方法以盐酸药根碱为对照品,采用紫外-可见分光光度法测定宽筋藤总生物碱的含量,测定波长为430nm。结果盐酸药根碱在0.001 1-0.006 5mg·mL-1范围内与吸光度呈良好线性关系,回归方程：Y=14.747 X-0.010 7（r=0.999 5）。盐酸药根碱平均回收率为100.4%,RSD=2.0%（n=6）。结论 16批宽筋藤药材总生物碱含量测定结果表明,所建立的方法简便、准确,可用于宽筋藤药材总生物碱的含量测定。
Objective To establish a method for the determination of total alkaloids in Tinospora sinensis caulis.Method The content of total alkaloids was determined,and hydrochloric jatrorrhizine was selected as a reference substance.Results Hydrochloric jatrorrhizine had a good linear relationship with the absorbance in the range of 0.001 1-0.006 5mg·mL^-1 and the regression equation was Y=14.747X-0.010 7（r=0.999 5）.The average recovery for hydrochloric jatrorrhizine was 100.4%,RSD=2.0%（n=6）.Conclusion The contents determination of the total alkaloids for sixteen batches of medicinal materials showed that the method was easy to operate and accurate,which could be used for the determination of the total alkaloids in Tinospora sinensis caulis.
出处
《西北药学杂志》
CAS
2015年第4期349-352,共4页
Northwest Pharmaceutical Journal
基金
北京中医药大学自主选题项目(2013-JYBZZ-XS-116)
关键词
宽筋藤
总生物碱
紫外-可见分光光度法
含量测定
Tinospora sinensis caulis
total alkaloids
UV-VIS spectrophotometry
content determination
