摘要
目的:对红毛七总生物碱提取工艺进行研究,为其大规模工业生产应用奠定基础。方法:以红毛七总生物碱中塔斯品碱为指标,建立塔斯品碱的HPLC分析方法,采用正交设计方法考察盐酸水浸提的最佳工艺,然后考察使用阳离子交换树脂分离红毛七总生物碱的工艺条件。结果:盐酸水浸提的最佳工艺为7倍量,1%盐酸,浸提3次。以2ml/min流速上LSD001阳离子交换树脂柱,10倍柱体积4%氨水乙醇为洗脱溶剂,红毛七总生物碱得率为1.35%,总生物碱中塔斯品碱含量为6.80%。结论:该方法工艺较简单,成本低,产率高。
Objective： To study the technological parameters of the extraction process of the total alkaloids from Caulopyhllum robstum. Methods： Taspine, which is main component of the total alkaloids from Caulopyhllum robustum, was selected as an evaluating marker and determined by HPLC. The orthogonal test was used to optimize extracting conditions in the process of acid water extraction. Then the optimized conditions for purification using cation exchange resin were investigated. Results： The optimized conditions in the process of acid water extraction were 1% hydrochloric acid as much as seven times of the medicine amount for 24hs and three times. Then the extraction of acid water was purified with a column of macroporous cation exchange resin LSD001 at 2 ml/min of flow rate,then eluted with 10BV of 4% aqueous ammonia ethanol. The extraction ratio of the total alkaloids was 1.35% and the content of taspine of the total alkaloids was 6. 80%. Conclusion ： This technology is simply, cheap effective and feasible for manufacture in great scale.
出处
《中药材》
CAS
CSCD
北大核心
2007年第2期220-223,共4页
Journal of Chinese Medicinal Materials
基金
陕西省科技攻关项目(No.2005K10-G1)
关键词
红毛七
总生物碱
阳离子交换树脂
工艺研究
Caulopyhllum robustum
Total Altaloids
Cation exchange resin
Technological study
