摘要
应用大孔吸附树脂和硅胶柱色谱制备高纯度辅酶Q_(10)。检测了7种大孔树脂对辅酶Q_(10)的吸附及解吸性能,确定HZ816为最佳吸附剂。工艺条件为:以无水乙醇为溶剂,上样浓度1.6～1.8 mg/ml,吸附流速3 BV/h,上样体积14 BV;分步洗脱:先用5 BV无水乙醇洗脱,流速3 BV/h;然后用丙酮洗脱。经初步纯化,辅酶Q_(10)纯度从56.5%提高到93.4%,回收率78.4%。利用硅胶柱色谱进一步纯化,获得纯度99%的辅酶Q_(10)。
Coenzyme Q 10 of high purity was obtained by using macroporous resins and silica gel chromatography. The adsorption and desorption characteristics of seven resins were tested and the resin HZ816 was selected as the best adsorbent. The separation condition of HZ816 was further optimized and the optimum conditions were as follows： the sample dissolved in absolute ethanol with concentration of 1.6 - 1.8 mg/ml was loaded into the resin HZ816 column for 14 BV loading amount at a rate of 3 BV/h and successively eluted with 5 BV of anhydrous ethanol and acetone. The eluent with coenzyme Q10 was concentrated and dried to obtain coenzyme Q10 which was purified from 56.5 % to 93.4 % purity, with a recovery of 78.4 %. The product was further purified by silica gel column chromatography with purity of 99 %.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2011年第12期894-898,共5页
Chinese Journal of Pharmaceuticals
关键词
辅酶Q10
大孔吸附树脂
硅胶柱色谱
纯化
coenzyme Q10
macroporous resin
silica gel column chromatography
purification
