摘要
建立一种同时快速分离测定不同品种、不同部位淫羊藿中双藿苷A、淫羊藿属苷A、朝藿定C、淫羊藿苷、淫羊藿属苷C、意卡瑞苷A和去多甲基羟基淫羊藿素7种异戊烯基黄酮类成分的梯度洗脱方法,其中双藿苷A、意卡瑞苷A和去多甲基羟基淫羊藿素为首次报道其含量测定。实验比较了三种不同的梯度洗脱条件和三种不同的提取分离条件,选出了实验的最佳条件:采用RP-HPLC法,以美国WATERS SUNFIRETM-C18为色谱柱,乙腈-水为流动相,梯度洗脱(乙腈:0 min,20%;10 min,40%;15 min,45%;20 min,60%),流速为1 mL/min,检测波长270 nm,在此条件下,30 m in内所有组分均得到了良好的分离。实验同时考察比较了巫山淫羊藿植株不同药材部位和其它十种不同品种淫羊藿中7种异戊烯基黄酮类成分的分布。结果表明:不同品种淫羊藿中黄酮类成分的含量差异很大,但大部分品种以朝藿定C为主;同一品种巫山淫羊藿不同部位中则双藿苷A和朝藿定C的含量较大(在巫山淫羊藿根中,两者的含量分别为现行质量控制指标淫羊藿苷的20倍和30倍),可见巫山淫羊藿显著不同于其它品种的淫羊藿,朝藿定C在其药用效果中可能比淫羊藿苷扮演着更重要的角色尤其是在根部作为药用时,这也为民间将巫山淫羊藿根作为小黄连使用提供了一定的实验支持。结论:对于品种较多,分布资源较广的淫羊藿药材,作为药用时,应注意品种和药用部位,考察药材的内在质量,不能一概而论。实验所得到的方法能被用做淫羊藿植株中二次代谢产物分布的快速考察、筛选符合要求的淫羊藿原药材和淫羊藿制备药物中的质量考察。
A simple and rapid gradient reverse-phase HPLC method for separation and simultaneous determination of the seven prenylflavones,diphylloside A,epimedoside A,Epimediin C,icariin,epimedoside C,icarisoside A,and desmethylanhydroicaritin in different organs（roots,stems,leaves） of Epimedii wushanense for the first time has been developed.Especially,diphylloside A,icarisoside A and desmethylanhydroicaritin（never found any reports of the detection by HPLC） were determined in Epimedii for quality control in the first time,comparison of three RP-HPLC methods and three extraction methods,quantification and comparison of each prenylflavone in leaves of Epimedii wushanense with other nine Epimedii species.With this method,we obtained good retention times and resolution of seven Compounds.Concentration of all seven components varied between plant organs,Epimediin C was higher than icariin（the Compound typically used to evaluate Epimedii quality） in leaves of Epimedii.diphylloside A and Epimediin C were 20 and 30x higher,respectively,than icariin in the roots of E.wushanense T.S.Ying,and the quantification of Epimediin C was higher far away from other Epimedii species.Thus,Epimedii wushanense is a special species of Epimedii,Epimediin C is likely to play a more important role than icariin in Epimedii wushanense,especially,roots of Epimedii wushanense should be exploit（it has been used to therapydisease as well as Coptidis phizoma in china in civilian）.The method could be applied to seek regularity in secondary metabolites distribution,screen raw materials in search for highly quality plants and its medical preparations.
出处
《天然产物研究与开发》
CAS
CSCD
2010年第5期820-825,共6页
Natural Product Research and Development
基金
安康学院高层次人才启动经费资助项目(AYQDZR200801)
