摘要
对马尾松嫩枝扦插苗生根特性进行观测,研究其生根过程中相关氧化酶(PPO,POD,IAAO)活性、黄酮类物质和营养物质等內源物质的动态变化。结果表明:马尾松属于愈伤组织生根类型,用适宜含量的生根剂GGR6处理马尾松插穗能显著提高其生根率和根系质量,且能改变內源物质的变化动态。在马尾松嫩枝扦插过程中,大部分內源物质均成规律性变化,其中PPO和IAAO活性在愈伤组织诱导期上升,在愈伤组织形成期达到高峰,在根系形成期下降;POD活性在愈伤组织形成期和根系形成期均出现高峰。內源黄酮类物质含量在扦插初期逐渐下降,30～50天又急剧升高,在愈伤组织大量形成期达到高峰,然后又逐渐下降;且黄酮含量的变化与过氧化物酶活性变化有关,过氧化物酶活性的高峰值总是对应着黄酮含量的最低值。可溶性糖含量在扦插后逐渐升高,插穗大量生根时达到高峰值,然后又逐渐下降。总氮含量在整个扦插过程中变化规律不明显,且变化幅度很小。
The rooting characteristics of the twig cuttings of Pinus massoniana was observed to investigate the dynamic changes of relevant endogenous components during rooting process,including the activity of related oxidative enzymes（PPO,POD,IAAO） and the content of flavonoid and nutritive substances and so on.Results showed that rooting of Pinus massoniana was derived from the callus.When its cutting was treated by root stimulate GGR6 in a suitable concentration,rooting rate and the quality of root system could be significantly enhanced.Moreover,dynamic state of the endogenous components in Pinus massoniana plantlets can also be changed.Changes of the most endogenous components displayed regular pattern during the cutting.The activity of PPO and IAAO increased during induction period of the callus,reached to a peak during the formation period of callus,and started to decrease during the formation of root system.Whereas the activity of POD reached to a peak in the formation of both root systems and callus.The content of endogenous flavonoid decreased gradually at early stage of cutting,increased sharply 30-50 days later and reached to a peak when callus formed considerably,and then it started to decrease gradually.The content changes of flavonoid had relationship with the activity of peroxidase（POD）;peak value of the activity of POD was always accompanied by a sharp decrease of the content of flavonoid.The content of soluble sugar increased gradually after cutting,reaching to a peak when most cuttings rooted,and then it decreased gradually.The content of total nitrogen changed irregularly during the whole process and the amplitude of variation was very small.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2010年第9期28-33,共6页
Scientia Silvae Sinicae
基金
公益性行业科研专项(201003044
201004039)
重庆市科技攻关重点项目(CSTC2009AB1115)
国家科技攻关项目(2004BA604A05)
重庆市自然科学基金重点项目(CSTC-2008BA7032)
重庆市林业科研项目"马尾松优良无性系引种及繁育技术研究"(411189)
西南大学生态学重点学科"211工程"建设项目
关键词
马尾松
生根特性
氧化酶
黄酮类物质
营养物质
Pinus massoniana
rooting characteristics
oxidases
flavonoids
nutrients
