摘要
选用品种(品系)、生长素(IBA、α-NAA)、质量分数3个因素,采用L16(42×29)正交设计研究榛子嫩枝扦插繁殖技术,研究生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)的活性变化。结果表明:生长素质量分数对扦插生根率影响最大,依次为品种、生长素种类。不同质量分数水平间生根率差异极显著,IBA0.03%质量分数处理的插穗生根率最高。不同品种间生根指数差异显著,巴赛罗娜生根指数最大。榛子嫩枝扦插生根过程可分为愈伤组织形成期、诱导期和表达期3个阶段。POD活性在愈伤组织形成期呈上升趋势,表达期下降;PPO活性在愈伤组织形成期和诱导期上升,表达期下降;IAAO活性在愈伤组织形成期上升,诱导期和表达期下降。
The cutting propagation technique and relevant oxidase changes of Corylus heterophylla were studied with a L16（4^2 ×2^9） orthogonal experiment design which consisted of three factors of varieties, auxins（IBA, a-NAA） and their concentrations. The activities of peroxidase （ POD ）, polyphenol oxidase （PPO） and indoleacetic acid oxidase （IAAO） during rooting were investigated in this paper. The results showed that the cutting rooting rate was mainly affected by auxin mass fraction, following by the variety and the auxin type among the tested factors. Percentage of rooting reached to maximum under 0.03% of IBA. Various varieties had significant effect on rooting index, with Barcelona the maximum. The adventitious rooting was obtained in three distinct phases i.e. callus formation, induction and expression. The peroxidase activity in IBA-treated cuttings increased in the callus formation phase and declined in the expression phase. Polyphenol oxidase activity in IBA-treated cuttings increased during callus formation and induction phase, however declined slowly during expression phase. Indoleacetic acid oxidase activity of IBA-treated cuttings increased during callus formation phase and decreased during induction and expression phases.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2008年第6期60-65,共6页
Scientia Silvae Sinicae
基金
国家“十五”重点攻关项目“仁用杏榛子优良品系筛选与丰产栽培技术研究”(2004BA515B12)
关键词
榛子
扦插生根
氧化酶
Corylus heterophylla
rooting of cuttings
oxidases
