摘要
以欧榛品种巴塞罗娜为材料,进行基质和生长素的对比试验,研究生根过程中过氧化物酶(POD)、多酚氧化酶(PPO)和吲哚乙酸氧化酶(IAAO)酶活性的动态变化。结果表明:在基质试验中,只有混合基质能够提高插穗生根率;在生长素试验中,IBA处理的插穗生根率达到60%,α-NAA和ABT1处理的插穗生根率只有15%和20%,而对照不生根。榛子硬枝扦插生根过程可分为诱导期、表达期和伸长期3个阶段。POD活性在诱导期和表达期呈上升趋势,伸长期下降,在第20天和60天各有1个高峰;PPO活性在诱导期和表达期上升,伸长期下降;IAAO活性在诱导期缓慢上升,表达期急剧上升,伸长期下降。
Cuttings propagation technology and related oxidase changes of Corylus avellana Barcelona were studied with media and auxin comparison experiments. The dynamic change of activities of peroxidase （ POD）, polyphenol oxidase （PPO） and indoleacetic acid oxidase （IAAO） during rooting were also investigated. The results showed that only the mixed media can enhance the percentage of cuttings rooting in the media experiment. Rooting percentage of cutting with 200 × 10^-6 IBA treatment was 60%, rooting percentage of cutting with 200 × 10^-6 α-NAA or ABT1 treatment was 15% and 20%, the cuttings of control did not root. The adventitious rooting displayed three distinct phases i.e. induction, expression and elongation. The peroxidase activity in IBA-treated cuttings increased during induction and expression phase declined in the elongation phase. There were two peaks of the POD activity respectively on the 20th and 60th during rooting, Polyphenol oxidase activity in IBA-treated cuttings increased during induction phase, and then declined slowly during expression and elongation phases. Indoleacetic acid oxidase activity of IBA-treated cuttings increased slowly during induction phase and then increased sharply during expression phases and decreased during elongation phases.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第12期70-75,共6页
Scientia Silvae Sinicae
基金
国家十五重点攻关项目"仁用杏榛子优良品系筛选与丰产栽培技术研究"(2004BA515B12)
关键词
欧榛
硬枝扦插
生根
氧化酶活性
Corylus avellana
hardwood cutting
rooting
oxidases
