摘要
目的:建立以高效液相色谱法测定肠炎清合剂中延胡索乙素含量的方法。方法:采用Hypersil ODS2 C_(18)柱色谱柱(250 mm×4.6 mm,5μm),流动相:甲醇-水-三乙胺(65:35:0.5),检测波长为280 nm,柱温为35℃;流速为1 ml·min^(-1)。结果:延胡索乙素在10～100μg·ml^(-1)范围内与峰面积积分值呈良好线性关系(r=0.999 9);平均加样回收率为96.81%,RSD=1.5%(n=6)。结论:本法快速、准确,重复性好,可用于肠炎清合剂的质量控制。
Objective： To determine the content of tetrahydropalmatine in changyanqing mixture by HPLC. Method： The Hypersil ODS2 C18 column （250 mm ×4.6 mm,5 μm） was used. The mobile phase consisted of methanol-water-triethylamine （65： 35： 0. 5 ） ; The flow rate was 1.0 ml·min^-1 and the detection wavelength was 280nm and the column temperature was 35 ℃. Result： The linear range of tetrahydropalmatine was 10-100 μg.ml^-1 （r =0. 999 9） and the recoverable rate was 96. 81% ,RSD = 1.5% （n =6）. Conclusion： The method is rapid, accurate and good repeatability, which can be used as the qualitative control of changyanqing mixture.
出处
《中国药师》
CAS
2010年第12期1760-1761,共2页
China Pharmacist
