摘要
目的观察苦参碱对黑色素瘤细胞株A375增殖的抑制作用。方法应用MTT比色法检测不同浓度苦参碱对A357细胞的增殖抑制,应用流式细胞仪观察苦参碱诱导A375细胞凋亡,采用透射电镜观察瘤细胞形态学改变。结果0.5g/L以上浓度的苦参碱对A375细胞增殖具有显著抑制作用,并与剂量相关。细胞凋亡率随着药物浓度的增大而增加。1.0g/L处理细胞24h后可见胞体明显变圆,透射电镜下发现细胞皱缩,染色质边集等细胞凋亡改变。结论苦参碱对黑色素瘤细胞株A375增殖有明显抑制作用,并能诱导其发生凋亡。
【Objective】To investigate the effects of matrine on the proliferation of human malignant melanoma cell line A375.【Methods】MTT [3-（4, 5）-dimethylthiahiazo （-z-y1）-3, 5-di-phenytetrazoliumromide] method was used to measure the levels of the proliferation of A375 cultured with matrine in different concentrations. The apoptosis rates of treated tumor cells were tested by FCM. The micro morphology of apoptotic tumor cells was observed by light microscopy and electronic microscopy.【Results】The proliferation of A375 was obviously inhibited by matrine which was more than 0.5 g/L in concentration in a dose-dependent manner. The inhibitory rate and ratio of apoptotic cells increased as the drug concentration elevated. The morphological changes of apoptosis were found by light microscopy and electronic microscopy, shown as enlargement of cell body and condensation of chromatin. 【Conclusion】Matrine can inhibit the proliferation of human malignant melanoma cell line A375 and induce the apoptosis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2010年第5期676-678,共3页
China Journal of Modern Medicine
