摘要
[目的]研究氯化镉对发育中的小鼠睾丸、肾、肝等脏器系数及对睾丸生殖细胞线粒体DNA控制区(D-Loop)基因突变的影响,探索氯化镉对睾丸影响的机制。[方法]取7周龄雄性ICR小鼠40只[体重(19.5±2.5)g],随机分为低(1μmol/kg)、中(5μmol/kg)、高(10μmol/kg)剂量氯化镉腹腔注射染毒组和阴性对照组共4组,每组10只,隔天染毒,共10次,对照组腹腔注射等体积的生理盐水。第21天取小鼠双侧睾丸、肾和肝,测定脏器系数;提取睾丸生殖细胞基因组DNA,聚合酶链反应(PCR)扩增线粒体D-Loop基因,纯化后测序分析基因突变。[结果]高剂量组睾丸脏器系数明显低于对照组(P<0.001),但肝脏器系数明显高于对照组(P<0.05);低剂量组肾脏脏器系数明显高于对照组(P<0.001)。测序结果显示各组小鼠睾丸生殖细胞线粒体D-Loop基因未检测到突变。[结论]高剂量的氯化镉对小鼠睾丸和肝脏脏器系数产生影响,低剂量的氯化镉对小鼠肾脏脏器系数产生影响。氯化镉处理小鼠21d,未检测到睾丸生殖细胞线粒体D-Loop基因突变。
[Objective] To study organ weight coefficient and testicle mitochondrial（mt）D-Loop point mutation in mice after exposure to cadmium.[Methods] Fourty male mice were divided into 4 groups with 10 animals each group（average body weight：19.5±2.5 g;age：7 week）.Three groups treated with intraperitoneal cadmium chloride of 1.0,5.0,10.0 μmol/kg,once per 2 days,10 times in total,and normal saline for control group.Necrospy were conducted 20 days after exposure of cadmium chloride,weight coefficients of testis,kidneys,liver were determined.The mt D-Loop gene was amplified by polymerase chain reaction（PCR）from the genomic DNA of mice testicle tissue,and their mutant sites by DNA sequencing was analyzed.[Results] The testis weight coefficient of 10.0 μmol/kg cadmium chloride group was lower than that of the control group（P 0.001）,but the liver weight coefficient of 10.0 μmol/kg cadmium chloride group was higher than that of the control group（P 0.05）.The kidney weight coefficient of 1.0 μmol/kg cadmium chloride group was higher than that of the control group（P 0.001）.No mt D-Loop mutation was found in the mice testicle spermatogenic cell.[Conclusion] High dose cadmium chloride affected the mice testicle and liver weight coefficient,low dose cadmium chloride affected that of kidney,but 21 day exposure did not induced mt D-Loop mutation.
出处
《环境与职业医学》
CAS
北大核心
2010年第11期695-697,共3页
Journal of Environmental and Occupational Medicine
基金
温州医学院2008年本专科学生科研申请立项自然科学类课题(编号:200801010)
