摘要
目的探讨纳米氧化石墨烯(nano-GO)对小鼠肝脏的急性氧化损伤作用。方法将80只健康清洁级ICR小鼠随机分为对照(高纯水)组和0.35 mg/kg(1/16 LD50)、0.70 mg/kg(1/8 LD50)、1.40 mg/kg(1/4 LD50)nano-GO染毒组,每组20只,雌雄各半。采用1次性尾静脉注射染毒,染毒容量为10 ml/kg。分别于注射3、15 d后,检测小鼠肝脏组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量。结果染毒3 d后,与对照组比较,各剂量nano-GO染毒组雌性小鼠和0.35 mg/kg nano-GO染毒组雄性小鼠肝脏的MDA含量及各剂量nano-GO染毒组小鼠肝脏的GSH-Px活力以及0.35、0.70 mg/kg nano-GO染毒组雄性小鼠肝脏的SOD活力均升高,0.70、1.40 mg/kg nano-GO染毒组雄性小鼠肝脏MDA含量和0.35、0.70 mg/kg nano-GO染毒组雌性小鼠肝脏的SOD活力均下降,差异均有统计学意义(P<0.05)。染毒15 d后,与对照组比较,各剂量nano-GO染毒组小鼠肝脏的SOD活力升高,0.70 mg/kg和1.40 mg/kg nano-GO染毒组小鼠肝脏的GSH-Px活力下降,差异均有统计学意义(P<0.05);而各剂量nano-GO染毒组小鼠肝脏的MDA含量均无明显改变。结论一次性尾静脉注射nano-GO后,肝脏可产生脂质过氧化作用,干扰小鼠肝脏抗氧化酶的活力。
Objective To study the acute oxidative damage in liver of mice exposed to nano-graphene oxide（nano-GO）.Methods Eighty clean ICR mice were randomly divided into four groups：the control group（highly purified water）,the nanoGO exposed groups with different doses of 0.35 mg/kg（1/16 LD50）, 0.70 mg/kg（1/8 LD50）, 1.40 mg/kg（1/4 LD50） body weight,20 in each group,through single tail vein injection. Then the levels of malondialdehyde（MDA）,superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in liver tissue homogenate were measured after 3-day and 15-day exposure to nano-GO respectively. Results Three days after the exposure,compared with the control group,the MDA contents were higher in liver of female mice in nano-GO exposure groups and in liver of male mice in 0.35 mg/kg nano-GO exposure group,the GSH-Px activities in liver of mice in nano-GO exposure group and the SOD activities in liver of male mice in 0.35, 0.70 mg/kg nanoGO exposure group were increased, the MDA contents were lower in liver of male mice in 0.70, 1.40 mg/kg nano-GO exposure groups, the SOD activities in liver of female mice in 0.35, 0.70 mg/kg nano-GO exposure groups were increased, the above differences were statistically significant（P〈0.05）. A total of 15 days after the exposure, compared with the control group, the SOD activities in liver of mice in nano-GO exposure group were increased,the GSH-Px activities in liver of mice in 0.70, 1.40mg/kg nano-GO exposure groups were decreased,the differences were statistically significant（P〈0.05）, the MDA contents in liver of mice in nano-GO exposure group had no significant change. Conclusion Nano-GO can induce lipid peroxidation and interfere with the activity of antioxidant in liver of mices.
出处
《环境与健康杂志》
CAS
北大核心
2015年第7期595-598,共4页
Journal of Environment and Health
关键词
纳米颗粒
氧化石墨烯
氧化损伤
Nanoparticals
Graphene oxide
Oxidative damage
