摘要
目的通过实验测定胸水中异烟肼的浓度,提高胸水中的药物浓度,增强抗结核作用。方法于2009年2月-6月采用反相高效液相色谱法测定异烟肼在结核患者胸水中的浓度。色谱柱：Agilent ZORBAX SB-C18（5μm,4.6 mm×2.5 mm）,进样20μL;柱温：室温;柱压200×105 Pa;流动相：甲醇（色谱纯）︰高纯水=20︰80;流速：1 mL/min;紫外检测波长：254 nm。结果异烟肼的检测浓度在0.25～10.00μg/mL范围内线性关系良好（r=0.998 9）;其低、中、高3种浓度（0.5、5.0、10.0μg/mL）的回收率分别为97.95%、100.64%、102.84%;日内相对标准偏差（RSD）分别为3.49%、1.45%、2.03%,日间RSD分别为3.85%、5.68%、4.15%。结论采用反相高效液相色谱法测定异烟肼在结核患者胸水中的浓度准确、灵敏度高、重现性好。
Objective To determinate the concentration of isonicotinyl hydrazide（INH） in the hydrothorax and thereby increase the drug concentration of the hydrothorax to enhance the anti-tuberculosis efficacy through experiments. MethodsBetween February and June 2009, we used high performance liquid chromatography（HPLC） to determinate the concentration of INH in the hydrothorax of tuberculosis（TB） patients. The separation of sample was performed on Agilent ZORBAX SBC18（5 μm, 4.6 mm×2.5 mm） column with a fixed sample injection volume of 20 μL. The mobile phase was methanol-water（20︰80） at a flow rate of 1 mL/min. The ultraviolet detective wavelength was set at 254 nm; The column temperature was room temperature. Results In the range of 0.25-10.00 μg/mL（r=0.998 9）, moxi-floxacin showed a good linear relation in HPLC. The recoveries of moxi-floxacin at three concentrations（0.5, 5.0, and 10.0 μg/mL） were 97.95%, 100.64%, and 102.84%, respectively, with intra-day relative standard deviation（RSD） at 3.49%, 1.45%, and 2.03%, respectively and intraday RSD at 3.85%, 5.68%, 4.15%, respectively. Conclusion The measuring method adopted in the experiment can accurately determinate the concentration of INH in the hydrothorax with high sensitivity and exceptional reproducibility.
出处
《华西医学》
CAS
2014年第8期1483-1486,共4页
West China Medical Journal
关键词
异烟肼
高效液相色谱法
结核患者
胸水
Isonicotinyl hydrazide
High performance liquid chromatography
Tuberculosis patients
Hydrothorax
