摘要
目的探讨耐甲氧西林金黄色葡萄球菌的辅助基因femA是否存在调控基因,以进一步揭示其耐药机制。方法用最低抑菌浓度法(MIC)筛选对苯唑西林高耐株、低耐株、敏感株;用头孢色芬纸片法(Nitrocephin)筛选出不产β-内酰胺酶菌株;用PCR筛选出含mecA的菌株。将含mecA不产酶的高耐株、低耐株、敏感株纳入进一步研究中;PCR扩增femA及femA5?上游基因;提取细菌总蛋白;生物素标记DNA探针;滞留电泳检测调控基因。结果滞留电泳检测对苯唑西林高度耐药不产β-内酰胺酶含mecA基因菌株均出现滞留条带。结论耐甲氧西林金黄色葡萄球菌的辅助基因femA有调控基因存在。
Objective To research the regulating gene of femA in methicillin-resistant Staphytococcus aureus.Methods Strains of Staphytococcus aureus were distinguished as high-resistant,low-resistant and sensitive to oxacillin with MIC.Strans of non-producedβ-lactamase were selected with Nitrocephin.Strains with mecA were selected with PCR.The regulating gene was detected with electrophoretic Mobility Shift Assay（EMSA）.Results All MRSA of no express β-lactamase had mecA gene Conclusion The regulating gene of femA probably lies in the 250 bp upstream sequence in MRSA.
出处
《西部医学》
2010年第11期1983-1985,共3页
Medical Journal of West China
基金
国家自然科学基金资助项目(NO.3990675)
国际合作CMB资助项目(NO.00722)
