摘要
目的比较不同形态乌药根中水、醇浸出物、乌药内酯、乌药醚内酯、异乌药内酯的含量。方法热浸法;色谱柱为Kromasil C18柱(250mm×4.6mm,5μm),流动相:甲醇-乙腈-水(35∶25∶40);检测波长:235nm;柱温:40℃;流速:1ml/min。结果乌药纺锤根中水、醇浸出物最高,乌药老根最低;而乌药内酯、乌药醚内酯、异乌药内酯的含量在乌药纺锤根中分别为1.10mg/g、1.51mg/g、3.10mg/g,乌药直根中为0.90mg/g、1.34mg/g、3.36mg/g,乌药老根中为0.55mg/g、0.78mg/g、1.40mg/g;乌药直根和乌药老根高效液相色谱图中有4号峰,具体成份尚不明确。结论不同形态乌药根质量存在一定差异,乌药直根能否纳入药用范围值得进一步探讨。
Objective To determine of water extract、alcohol extract、linderalactone、linderane and isolinderalactone in different forms linderae radix.MethodsHot-dip method;The sample was separated on Kromasil C18（250 mm×4.6 mm,5 μm）column,the mobile phase was ethanol-acetonitrile-water（35∶25∶40）at a flow rate of 1.0 ml/min and the detection wavelength was 235 nm,and the column temperature was 40 ℃.ResultsWater and alcohol extract was the maximum in spindle-shaped linderae radix.It was the minimum in Old linderae radix.The content of linderalactone、linderane and isolinderalactone was 1.10 mg/g、1.51 mg/g and 3.10 mg·g 1 in spindle-shaped linderae radix,it was 0.90 mg/g、1.34 mg/g and 3.36 mg/g in straight linderae radix,it was 0.55 mg/g、0.78 mg/g and 1.40 mg/g in old linderae radix.There was the 4th peak in HPLC of Old linderae radix and straight linderae radix,but specific component was not clear.ConclusionThere were quality difference in different forms linderae radix.It can worthy of further approach whether straight linderae radix is ranged into the medicine.
出处
《中国现代药物应用》
2010年第19期1-3,共3页
Chinese Journal of Modern Drug Application
关键词
乌药老根
乌药直根
乌药纺锤根
乌药内酯
乌药醚内酯
异乌药内酯
Old linderae radix
Straight linderae radix
Spindle-shaped linderae radix
Linderalactone
Linderane
Isolinderalactone
