摘要
目的 研究桑黄提取物对小鼠S180实体瘤、H22肝癌和Lewis肺癌实体瘤的生长抑制作用,体外对人瘤细胞的直接抑制作用.方法 建立小鼠S180实体瘤、H22肝癌和Lewis肺癌实体瘤模型,观察桑黄提取物高（1000mg/kg）、中（500mg/kg）、低（250mg/kg）剂量组体内抑制肿瘤生长作用,MTT法检测桑黄提取物抑制人肿瘤细胞增殖的作用.结果 ①桑黄提取物高、中、低剂量对小鼠S180实体瘤的抑制作用分别为27.9%、75%、31.2%;对小鼠H22肝癌的抑制率分别为46%、63%、57%;对小鼠肺癌Lewis的抑制率分别为57.1%、54.5%、45.9%.②体外对人瘤细胞的抑制作用：桑黄提取物给药剂量（20 μg/ml、40μg/ml、80μg/ml、160 μg/ml）对人卵巢癌细胞（A2780）的抑制率分别为16.9%、18.5%、25.8%、31.5%;对人乳腺癌（MCF7）细胞的抑制率分别为19.9%、28.1%、32.25%、30.8%.结论 桑黄提取物各剂量组对小鼠H22、S180、Lewis实体型肿瘤的生长均有抑制作用,体外对人肿瘤细胞MCF7,OVCA278有直接杀伤作用.
Objective To study the inhibiting effects of phellinus igniarius extractive （PLE） on tumor growth of tumor-bearing mice in vivo, and the direct inhibiting action to human tumor in vitro. Methods ①Models of hepatoma,sarcoma, lung cancer in mice were established. Experiments in studying the effect of PLE on the above three animal models were repeated for three times. ②The suppression of cellular growth of human tumor was detected by MTT assay. Results PLE （250.500 and 1000 mg/kg, ig, d1 to d11） could inhibit the growth of H22 tumor cells in mice at the inhibitory rate of 57%,63% and 46%,respectively; slow down the growth of S180 tumor cells in mice at the inhibitory rate of 27.9%, 75%, and 31.2%,respectively; suppress the growth of Lewis lung cancer in mice at the inhibitory rate of 57.1%, 54.5%, 45.9%; and hold back the proliferation of MCF7 cells and OVCA2780 cells （P〈0.01）. Conclusion PLE （250.500 and 1000 mg/kg, ig, d1 to d11）could inhibit the growth of H22 tumor cells, S 180 tumor cells, and Lewis lung cancer in mice. PLE could significantly reduce the proliferation of MCF7 cells and OVCA2780 cells （P〈0.05）.
出处
《国际中医中药杂志》
2010年第5期391-393,共3页
International Journal of Traditional Chinese Medicine
