摘要
目的:探讨双氢青蒿素对小鼠Lewis肺癌移植瘤的抑瘤效应及其作用机制。方法:50只C57BL/6J小鼠皮下接种3LL细胞(2×106),随机分为5组,分别为生理盐水组,阳性对照顺铂组,双氢青蒿素高、中、低剂量组(150、100、50mg/kg),检测各组小鼠的体重变化和抑瘤率,应用流式细胞术进行瘤细胞的DNA倍体分析。结果:双氢青蒿素中、高剂量组体重无生理盐水组增加明显,其抑瘤率分别为53.50%及59.24%;流式细胞术检测双氢青蒿素能诱导肿瘤细胞凋亡,并能影响肿瘤的细胞周期,G0/G期及G2/M期细胞数大量减少,细胞被阻滞在S1期。结论:口服双氢青蒿素对Lewis小鼠肺癌有明显的抑制作用,可促进肿瘤细胞凋亡。
AIM： To explore the anti-tumor effect and the mechanism of dihydroarteannuin in C57BL/6J mice with lewis lung cancer. METHODS： Fifty C57BL/6J mice subcutaneously planted with 3LL lung cancer cells （2 × 10^6 ） were randomly divided into 5 groups： blank control group, same volume of normal saline group, positive control DDP group, low, middle and high dose dihydroarteannuin groups. Changes of body weight and inhibitory rate of tumor in each group were observed. The cell cycle and apoptosis rates were analyzed by flow cytometry. RESULTS： The body weights were decreased in middle and high dose group compared with NS group and the inhibitory rate of tumor were 53.50% and 59.24% respectively. FCM as- say indicated that Dihydroarteannuin could induce apoptosis of lung cancer cell. At the same time, the number of G0/G1 and G2/M phase cells was decreased. Most of the cells were arrested in S1 phase. CONCLUSION： Dihydroarteannuin has obviously effect on anti-tumor in C57BL/6J mice with lung cancer. Its possible mechanism might be involved in inducing cancer cell apoptosis.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2008年第8期891-894,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
