摘要
为评价桑黄Sanghuangporus sanghuang子实体醇提物对SW620结肠癌细胞的影响,用alamarBlue?法测定细胞增殖率,用流式细胞术碘化丙啶(propidim iodide,PI)染色法和2′,7′-dichlorofluorescin diacetate (H2DCFDA)染色法分别检测细胞早期凋亡率、细胞周期变化和活性氧(reactive oxygen species,ROS)释放量,结果表明桑黄子实体醇提物在12.5–100μg/mL作用浓度下具有抑制SW620细胞增殖的作用,但对中国仓鼠卵巢(Chinese hamster ovarycell,CHO)细胞和小鼠骨髓巨噬细胞的增殖无显著抑制作用;桑黄子实体醇提物能诱导SW620细胞凋亡,引起细胞周期变化,可降低G0/G1和G2/M期细胞数量,并呈现浓度梯度依赖性,ROS实验结果提示桑黄子实体醇提物的促肿瘤细胞凋亡与ROS释放相关。
The inhibition effect on SW620 colon cancer cells of ethanol extract of Sanghuangporus sanghuang fruiting bodies was studied.The proliferation rate was determined by alamarBlue? method.The early apoptosis was determined by annexin V/Propidium iodide(PI)double staining and flow cytometry analysis.The cell cycle was determined by PI staining and flow cytometry analysis.Release of reactive oxygen species(ROS)by SW620 cells was determined by 2′,7′-dichlorofluorescin diacetate(H2DCFDA)probe and flow cytometry analysis.The results indicated that SW620 colon cancer cells treated with ethanol extract of S.sanghuang fruiting bodies were significantly inhibited at the concentration of 12.5-100μg/mL,while no cytotoxic effects on CHO cells and macrophages were detected.Flow cytometry analysis revealed that the ethanol extract of S.sanghuang fruiting bodies increased SW620 cell apoptosis,and decreased the number of cells in G0/G1 and G2/M phases of cell cycle in a concentration-dependent manner and the apoptosis was related to the release of ROS by SW620 cells.
作者
汪雯翰
杨焱
朱丽娜
贾薇
张劲松
刘艳芳
颜梦秋
赵香丽
张凯
张赫男
樊华
WANG Wen-Han;YANG Yan;ZHU Li-Na;JIA Wei;ZHANG Jing-Song;LIU Yan-Fang;YAN Meng-Qiu;ZHAO Xiang-Li;ZHANG Kai;ZHANG He-Nan;FAN Hua(Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungal Resources and Utilization (South), Ministry of Agriculture, P.R. China, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungal Processing, Key Laboratory of Agricultural Genetics and Breeding of Shanghai, Shanghai 201403, China;Institut für Laboratoriumsmedizin Klinische Chemie und Pathobiochemie, Campus Virchow Klinikum, Charite-Universit?tsmedizin, Berlin 13353, Germany)
出处
《菌物学报》
CAS
CSCD
北大核心
2018年第12期1751-1760,共10页
Mycosystema
基金
上海市市级农口系统青年人才成长计划[沪农青字(2018)第1-17号]
上海市农业科学院卓越团队[农科创2017(A-06)]
关键词
增殖
凋亡
细胞周期
活性氧
proliferation
apoptosis
cell cycle
reactive oxygen species
