摘要
目的:观察桑黄多糖对荷瘤小鼠的抑瘤效应并探讨其作用机理。方法:建立小鼠肝癌(H22)、肉瘤(S180)和肺癌(Lew-is)模型,测定桑黄多糖对小鼠肿瘤的抑制作用。通过对巨噬细胞(MΦ)功能和肿瘤坏死因子(TNF-α)的测定,探讨其对免疫功能的影响。结果:桑黄250、500、1000mg/kg 3个剂量组对S180、H22和Lew is肺癌均有明显的抑瘤作用,但无明显的量效关系;桑黄激活巨噬细胞的最小有效剂量为100μg/m l,桑黄还能促进小鼠巨噬细胞分泌肿瘤坏死因子,100μg/m l桑黄组产生的肿瘤坏死因子是对照组的4倍。结论:桑黄对H22、S180和Lew is肺癌均表现出较好的抑瘤作用,激活巨噬细胞、增强其吞噬功能、诱导巨噬细胞产生和分泌肿瘤坏死因子是桑黄抗肿瘤作用的重要机制之一。
Aim： To investigate the anti-tumor activities of crude polysaccharides （PL） from the mushroom Phellinus linteus on transplanted tumor in mice and observe the effects on the functions of peritoneal macrophages （PM） in vitro. Method： Anti-tumor activities were assayed by weighing tumor weight. LDH release assay was utilized for measuring PM-mediated lysis of tumor cell. The TNF-α secretion of PM was measured by Elisa assay. Result： PL at regimen 250, 500 and 1000mg/kg. day × 10 day inhibited evidently the development of transplanted tumor in mice, in vitro PL a dose 100μg/ml up-regulated PM-mediated cytotoxicity and enhances TNF-α secretion of PM in dose-dependent manner. Conclusion： PL inhibited evidently the development of transplanted tumor in mice, enhancing PM function and up-regulation of TNF-α tumor activity.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2006年第3期56-58,共3页
Pharmacology and Clinics of Chinese Materia Medica
