摘要
采用滤纸片富集法对采自广东省信宜的土壤样品进行了粘细菌的分离和纯化，共获得6个菌株（Soce M1～So ce M6）。利用体视显微镜、倒置显微镜和扫描电镜对这些菌株的子实体、菌落及营养细胞等进行了形态学的观察和特征描述。同时，还测定了6个菌株的16SrRNA基因序列，应用blast程序与GenBank中已知的纤维堆囊菌及其他粘细菌序列进行比对，并采用邻位相连法（Neighbor-Joining，NJ）构建了分离菌株与相关菌株的系统发育树。结果表明：6个菌株均为纤维堆囊菌（Sorangium cellulose），但这些菌株在形态特征和16S。RNA基因进化距离上存在着一定的差异，表现出纤维堆囊菌菌株的形态多样性和遗传多样性。
Six myxobacteria strains were isolated by a filter paper enrichment method from soil samples collected in Xinyi, Guangdong province. The morphological characteristics were provided based on microscopic observations of the fruiting bodies, swarms and vegetative cells of these strains using stereo microscope, inverted microscope and scanning electron microscope. The 16S rRNA genes of the isolated strains were sequenced and compared with those of Sorangium cellulose and other known myxobacteria from Gen- Bank. The phylogenetic tree was constructed by neighbor-joining method with the MEGA 4.1 program. The results showed that the 6 strains were all S. cellulose although there were some differences among them in morphological characteristics as well as in evolutionary distances of the 16S rDNA sequences, which also revealed intraspecific morphological and genetic diversities of S. celluloses.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2010年第4期387-393,共7页
Journal of Jilin Agricultural University
基金
广东省科学院优秀青年科技人才项目
关键词
粘细菌
纤维堆囊菌
分离
鉴定
16S
RRNA
myxobacteria
Sorangium cellulose
isolation
identification
16S rRNA
