摘要
粘细菌的次级代谢产物药用潜力巨大,粘细菌的分离纯化及鉴定对粘细菌的研究而言意义重大。常用的分离方法有食草动物粪便诱导法、滤纸诱导法和细菌划线法;纯化方法有直接纯化法和间接纯化法;鉴定方法除传统的镜检法外目前比较通用的是16S r DNA测序比对法。
The secondary metabolites of myxobacteria have great potential for medicinal use, and the separation, purification and identification of myxobacteria are of great significance in the study of myxobacteria.The commonly used methods of isolation are herbivore fecal induction,filter paper induction and bacterial streak. The purification methods include direct and indirect purification. In addition to the traditional identification methods of microscopy, another method is 16S rDNA sequencing method.
作者
孙宝婷
祝文兴
刘新利
SUN Bao-ting ZHU Wen-xing LIU Xin-li(School of Biological Engineering, Qilu University of Technology, Jinan 250353, Chin)
出处
《齐鲁工业大学学报》
2017年第4期25-28,共4页
Journal of Qilu University of Technology
基金
国家自然科学基金(21272139)
关键词
粘细菌
分离纯化
鉴定
myxobacteria
separation and purification
identification
