摘要
目的:建立以反相高效液相色谱法测定清肺抑火丸中白花前胡甲素含量的方法。方法:色谱柱为Diamonsil-C18(250mm×4.6mm,5μm),流动相为甲醇-水(81:19),检测波长为322nm。结果:白花前胡甲素的检测浓度在4.65～23.25μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为100.5%,RSD=2.35%(n=6)。结论:本方法分离效果好、灵敏、准确,可用于清肺抑火丸中白花前胡甲素的含量测定。
OBJECTIVE：To establish RP-HPLC method for the content determination of praeruptorin A in Qingfei yihuo pills.METHODS：The chromatographic column was Diamonsil-C18（250 mm×4.6 mm,5 μm） column.The mobile phase consisted of methanol-water（81：19） and the detection wavelength were set at 322 nm.RESULTS：The linear range of praeruptorin A was 4.65～23.25 μg·mL^-1（r=0.999 9） with an average recovery of 100.5%（RSD=2.35%,n=6）.CONCLUSION：The method is sensitive,well separated and accurate for content determination of Qingfei yihuo pills.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第27期2556-2557,共2页
China Pharmacy
