摘要
目的建立清肺抑火丸中盐酸黄柏碱的含量测定方法。方法采用离子对色谱法，Diamonsil—C18柱，乙腈-0．1％磷酸水溶液（31：69）（每100ml中加十二烷基磺酸钠0．18g）为流动相，流速为1．0ml·min-1，检测波长为284nm，柱温为30℃。结果盐酸黄柏碱浓度在5．6～28．0μg·ml-1间具有良好的线性关系（r=0．9996），盐酸黄柏碱平均回收率为99．4％，方法精密度（RSD）为0．71％（n=-6）。结论该法可用于清肺抑火丸中盐酸黄柏碱的含量测定。
Objective To establish a method for detecting phellodendrine hydrochloride content in Qingfeiyihuo pills. Methods Paired ion chromatography was used. Chromatographic column was diamonsil-Cls. Mobile phase was a mixture of acetonitrile - 0.1% phosphoric acid （ 31：69, V/V ）（ containing 1.8g sodium dodecyl sulfonate per 100 ml ）, with a flow rate of 1.0 mL·min-1. Detection wavelength was 284 nm. Results Phellodendrine hydrochloride had a better linear relation at a concentration of 5.6 - 28. 0μg·ml-1（ r = 0.9996 ）. The average recovery rate for phellodendrine hydrochloride was 99.4% ; precision of the method was 0.71% （ RSD, n = 6）. Conclusions This method can be used in detecting phellodendrine hydrochloride content in Qingfeiyihuo pills.
出处
《国际医药卫生导报》
2011年第19期2407-2410,共4页
International Medicine and Health Guidance News
