摘要
剖取由温和气单胞菌感染后死亡或发病的日本鳗鲡以及健康鳗鲡的鳃、肝脏、胃、肾脏及肠,分别测定5种组织中超氧化物歧化酶(SOD)的活性和脂质过氧化物丙二醛(MDA)的含量。研究结果表明,病死鳗鲡组和染病鳗鲡组的鳃、肝脏、胃、肾脏4种组织的SOD活性显著(P<0.05)低于健康鳗鲡组,而MDA含量显著(P<0.05)高于健康鳗鲡组,病死鳗鲡组和染病鳗鲡组的肠组织SOD活性、MDA含量与健康鳗鲡组相比差异不显著。温和气单胞菌可导致鳗鲡的鳃、肝脏、胃、肾脏等组织SOD活性降低,MDA含量升高,进而使其抗氧化功能降低,组织受损,甚至发病或死亡。
The author evaluated the activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA）with the method of chloride nitro tetrazolylazo blue （NBT）-reduction and thiobarbituric acid （TBA）, respectively. The gill, liver, stomach, kidney and intestines of dead or live Anguilla japonica were infected by Aeromonas sobria and the healthy Anguilla japonica.The results showed that the SOD activity of tissues including gills, liver, stomach and kidney in the dead and nosopoietic groups were significantly lower than that of the healthy group, and the MDA amounts were higher than that of the healthy group of Anguilla japonica （P0.05）, while the SOD activity and MDA amounts of intestines in the infected groups were not in significant difference. It also indicated that Aeromonas sobria was responsible for reduction of the SOD activity and enhancement the MDA amounts in the organizations of Anguilla japonica like gill, liver, stomach, kidney, which were related to the decrease of functions of antioxidation and induced the Anguilla japonica to be invalid or dead.
出处
《湖北农业科学》
北大核心
2010年第6期1434-1436,共3页
Hubei Agricultural Sciences
基金
广东省重大科技兴海(兴渔)项目(A200501G01)
广州市科技攻关项目(2006Z3-E0291)
关键词
日本鳗鲡
温和气单胞菌
抗氧化功能
超氧化物歧化酶
丙二醛
Anguilla japonica
Aeromonas sobria
antioxidation function
superoxide dismutase（SOD）
malondialdehyde（MDA）
