摘要
剖取由温和气单胞菌感染后发病的大口黑鲈及健康大口黑鲈的心脏、脾脏、肾脏、鳃、肌肉、肠、肝7种组织,分别采用氯化硝基四氮唑蓝(NBT)光还原法和硫代巴比妥酸(TBA)比色法测定组织中超氧化物歧化酶(SOD)的活性和脂质过氧化物丙二醛(MDA)的含量。研究结果表明,发病大口黑鲈的心脏、脾脏、肾脏、鳃、肝5种组织的SOD活性显著(P<0.05)低于健康大口黑鲈,而MDA含量显著(P<0.05)高于健康大口黑鲈。发病大口黑鲈的肌肉、肠组织SOD活性与MDA含量与健康大口黑鲈相比差异不显著。研究结果提示温和气单胞菌可导致大口黑鲈的心脏、脾脏、肾脏、鳃和肝等组织SOD活性降低,MDA含量升高,进而使其抗氧化功能降低,组织受损,甚至发病。
To evaluate the activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） with the method of chloride nitro tetrazolylazo Blue（NBT）-reduction and thiobarbituric acid （TBA）, respectively. The heart, spleen, kidney, gills, muscle, intestines and liver of the Micropterus salmoides were infected by Aeromonas sobria and the healthy Micropterus salmoides. The results showed that the SOD activity of tissues including heart, spleen, kidney, gills and liver in the nosopoietic groups were significantly lower than that of the healthy group, and the MDA amounts were higher than that of the healthy group of Micropterus salmoides （P〈0.05）, while the SOD activity and MDA amounts of muscle and intestines in the infected groups were not in significant difference. It also indicated that Aeromonas sobria was responsible for reduction of the SOD activity and enhancement the MDA amounts in the organizations of Micropterus salmoides like heart, spleen, kidney, gills, liver, which were related to the decrease of functions of antioxidation and induced the Micropterus salmoides to be invalid.
出处
《湖北农业科学》
北大核心
2009年第7期1719-1721,共3页
Hubei Agricultural Sciences
基金
广东省重大科技兴海(兴渔)项目(A200501G01)
广州市科技攻关项目(2006Z3-E0291)
关键词
大口黑鲈
温和气单胞菌
抗氧化功能
超氧化物歧化酶
丙二醛
Micropterus salmoides
A eromonas sobria
antioxidation function
superoxide dismutase（SOD）
malondialdehyde（MDA）
