摘要
目的:探讨一种有效记录人肠系膜动脉平滑肌细胞大电导钙激活钾通道电流的穿孔全细胞膜片钳方法,使细胞内环境保持稳定,改善常规全细胞膜片钳破膜所造成的封接不稳定。方法:以人肠系膜动脉平滑肌细胞为研究对象,采用穿孔全细胞膜片钳技术,记录人肠系膜动脉平滑肌细胞上大电导钙激活钾通道(big-conductance Ca2+-activated potassium channels,BKCa)宏观电流以及自发性瞬时外向电流(spontaneous transient outward currents,STOCs)。结果:采用这种穿孔全细胞膜片钳技术,能有效记录到人肠系膜动脉平滑肌细胞上BKCa和STOCs。结论:穿孔膜片钳技术是一种简便而有效的记录全细胞电流的实验方法,该方法的建立为今后更深入的研究提供实验基础。
Objective： To discuss an effective method of recording big-conductance calcium-activated potassium channel currents by perforated whole-cell patch clamp technique in human mesenteric artery smooth muscle cells,which makes cells to maintain a stable internal environment, to improve conventional whole-cell patchclamp instability caused by the rupture of membrane. Methods： In this experiment, big-conductance calcium-activated potassium channel macroscopic currents and spontaneous transient outward currents in human mesenteric artery smooth muscle cells were measured using by perforated whole-cell patch-clamp method. Results： Using our method, BKCa and STOCs in human mesenteric artery smooth muscle cells were effectively measured. Conclusion： Perforated whole-cell patch clamp technique is a simple and effective experimental method of recording whole-cell currents, and this method provides a more experimental basis for the future.
出处
《泸州医学院学报》
2010年第3期225-228,共4页
Journal of Luzhou Medical College
基金
国家自然科学基金资助项目(编号:30670763)
关键词
平滑肌细胞
大电导钙激活钾通道
自发性瞬时外向电流
穿孔全细胞膜片钳
Smooth muscle cells
Big-conductance calcium-activated potassium channel
Spontaneous transient outward currents
Perforated whole-cell patch clamp
